Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN1859155 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 218-506) antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant HIF1a (Thr218~Thr506) expressed in E.coli.
- Description
- Affinity Chromatography
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
The target protein is fused with tw
o N-terminal Tags, His-tag and T7-t
ag: MGSSHHHHHH SSGLVPRGSH MASMTGG
QQM GRGS-TCL VLICEPIPHP SNIEIPLDSK
TFLSRHSLDM KFSYCDERIT ELMGYEPEEL LG
RSIYEYYH ALDSDHLTKT HHDMFTKGQV TTGQ
YRMLAK RGGYVWVETQ ATVIYNTKNS QPQCIV
CVNY VVSGIIQHDL IFSLQQTECV LKPVESSD
MK MTQLFTKVES EDTSSLFDKL KKEPDALTLL
APAAGDTIIS LDFGSNDTET DDQQLEEVPL Y
NDVMLPSPN EKLQNINLAM SPLPTAETPK PLR
SSADPAL NQEVALKLEP NPESLELSFT MPQIQ
DQTPS PSDGST- Epitope
- AA 218-506
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- 200 μg/mL
- Storage
- Store at 4°C for frequent use. Stored at -20°C to -80°C in a manual defrost freezer for one year without detectable loss of activity.
- Handling
- Avoid repeated freeze-thaw cycles.
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Supportive validation
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- antibodies-online (provider)
- Main image
- Experimental details
- Image(s): Western Blotting