Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- IC1935P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal HIF-1 alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Recognizes human and mouse HIF-1 alpha in direct ELISAs.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Induction of HIF-1α by HIV-1 Infection in CD4+ T Cells Promotes Viral Replication and Drives Extracellular Vesicle-Mediated Inflammation.
Glucocorticoid receptor promotes the function of myeloid-derived suppressor cells by suppressing HIF1α-dependent glycolysis.
Duette G, Pereyra Gerber P, Rubione J, Perez PS, Landay AL, Crowe SM, Liao Z, Witwer KW, Holgado MP, Salido J, Geffner J, Sued O, Palmer CS, Ostrowski M
mBio 2018 Sep 11;9(5)
mBio 2018 Sep 11;9(5)
Glucocorticoid receptor promotes the function of myeloid-derived suppressor cells by suppressing HIF1α-dependent glycolysis.
Lu Y, Liu H, Bi Y, Yang H, Li Y, Wang J, Zhang Z, Wang Y, Li C, Jia A, Han L, Hu Y, Zhao Y, Wang R, Liu G
Cellular & molecular immunology 2018 Jun;15(6):618-629
Cellular & molecular immunology 2018 Jun;15(6):618-629
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of HIF-1 alpha in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line treated with CoCl2 was stained with Mouse Anti-Human/Mouse HIF-1 alpha PE-conjugated Monoclonal Antibody (Catalog # IC1935P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.