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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- TA309691 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67)
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HIF1A
- Isotype
- IgG
- Antibody clone number
- H1alpha67
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: HIF-1 alpha Antibody (H1alpha67) - Upon quantification, 100ug of MEF cells (+/+,-/-) protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at 1:500. The secondary antibody was conjugated with HRP and was used at 1:2500.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: HIF-1 alpha Antibody (H1alpha67) - Detection of HIF-1 alpha in cobalt chloride treated and untreated COS-7 nuclear extracts.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) - Heavy and light chains are also detected.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) - Staining of HIF-1 alpha in human kidney.
- Validation comment
- IHC
