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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Western blot [2]
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- Product number
- NBP1-02160 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-02160, RRID:AB_1503373
- Product name
- Rabbit Polyclonal HIF-1 alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Bovine, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references HIF1 driven transcriptional activity regulates steroidogenesis and proliferation of bovine granulosa cells.
Early-Stage Metastasis Requires Mdm2 and Not p53 Gain of Function.
Anti-apoptosis effects of vascular endothelial cadherin in experimental corneal neovascularization.
Plasma from human volunteers subjected to remote ischemic preconditioning protects human endothelial cells from hypoxia-induced cell damage.
An insert-based enzymatic cell culture system to rapidly and reversibly induce hypoxia: investigations of hypoxia-induced cell damage, protein expression and phosphorylation in neuronal IMR-32 cells.
Effect of alginate encapsulation on the cellular transcriptome of human islets.
Baddela VS, Sharma A, Michaelis M, Vanselow J
Scientific reports 2020 Mar 3;10(1):3906
Scientific reports 2020 Mar 3;10(1):3906
Early-Stage Metastasis Requires Mdm2 and Not p53 Gain of Function.
Hauck PM, Wolf ER, Olivos DJ 3rd, Batuello CN, McElyea KC, McAtarsney CP, Cournoyer RM, Sandusky GE, Mayo LD
Molecular cancer research : MCR 2017 Nov;15(11):1598-1607
Molecular cancer research : MCR 2017 Nov;15(11):1598-1607
Anti-apoptosis effects of vascular endothelial cadherin in experimental corneal neovascularization.
Liu GQ, Wu HY, Xu J, Wang MJ, Lu PR, Zhang XG
International journal of ophthalmology 2015;8(6):1083-8
International journal of ophthalmology 2015;8(6):1083-8
Plasma from human volunteers subjected to remote ischemic preconditioning protects human endothelial cells from hypoxia-induced cell damage.
Weber NC, Riedemann I, Smit KF, Zitta K, van de Vondervoort D, Zuurbier CJ, Hollmann MW, Preckel B, Albrecht M
Basic research in cardiology 2015 Mar;110(2):17
Basic research in cardiology 2015 Mar;110(2):17
An insert-based enzymatic cell culture system to rapidly and reversibly induce hypoxia: investigations of hypoxia-induced cell damage, protein expression and phosphorylation in neuronal IMR-32 cells.
Huang Y, Zitta K, Bein B, Steinfath M, Albrecht M
Disease models & mechanisms 2013 Nov;6(6):1507-14
Disease models & mechanisms 2013 Nov;6(6):1507-14
Effect of alginate encapsulation on the cellular transcriptome of human islets.
Vaithilingam V, Quayum N, Joglekar MV, Jensen J, Hardikar AA, Oberholzer J, Guillemin GJ, Tuch BE
Biomaterials 2011 Nov;32(33):8416-25
Biomaterials 2011 Nov;32(33):8416-25
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: HIF-1 alpha Antibody [NBP1-02160] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HIF-1 alpha Antibody [NBP1-02160] - Detection of HIF-1 alpha using NBP1-02160. Lane 1: COS7 CoCl treated cells Lane 2: COS7 untreated cells.
