NB100-123L

antibody from Novus Biologicals

Targeting: HIF1A bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

NB100-123L

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-123L, RRID:AB_2232953

Product name

Mouse Monoclonal HIF-1 alpha Antibody

Antibody type

Monoclonal

Description

Protein A purified.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Porcine, Rabbit, Sheep, Simian

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C in the dark.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HIF-1 alpha Antibody (H1alpha67) [HiLyte Fluor 488] [NB100-123L] - On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution (NB100-123). The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution. Photo courtesy of Dr. Gregg Semenza, Johns Hopkins University.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HIF-1 alpha Antibody (H1alpha67) [HiLyte Fluor 488] [NB100-123L] - Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) [HiLyte Fluor 488] [NB100-123L] - Immunoprecipitation of HIF-1alpha using NB100-123. Heavy and light chains are also detected.