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- Antibody Data
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- Immunoprecipitation [1]
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- Product number
- NBP2-69088 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal HIF-1 alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Methylation of hypoxia-inducible factor (HIF)-1α by G9a/GLP inhibits HIF-1 transcriptional activity and cell migration.
Bao L, Chen Y, Lai HT, Wu SY, Wang JE, Hatanpaa KJ, Raisanen JM, Fontenot M, Lega B, Chiang CM, Semenza GL, Wang Y, Luo W
Nucleic acids research 2018 Jul 27;46(13):6576-6591
Nucleic acids research 2018 Jul 27;46(13):6576-6591
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: HIF-1 alpha [Monomethyl Lys674] Antibody [NBP2-69088] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to immunoprecipitation with antibodies against K674me1 or K674me2, followed by immunoblot assays with antibodies against HIF-1 alpha, G9a or actin. (Image courtesy of Weibo Luo, UT Southwestern).
