A00013-3

antibody from Boster Biological Technology

Targeting: HIF1A bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8

Provider product page for A00013-3

Western blot

ELISA

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [26]
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Validations
Western blot [1]

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Product number: A00013-3 - Provider product page
Provider: Boster Biological Technology
Product name: Anti-HIF-1 alpha/HIF1A Antibody Picoband™
Antibody type: Polyclonal
Description: Rabbit IgG polyclonal antibody for HIF-1 alpha/HIF1A detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human;Mouse;Rat.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Vial size: 100μg/vial
Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Handling: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

HIF1A protein structure - A00013-3 shown in red.

Submitted references Combined Treatment of Tanshinone I and Epirubicin Revealed Enhanced Inhibition of Hepatocellular Carcinoma by Targeting PI3K/AKT/HIF-1α.

Zhao J, Lin E, Cai C, Zhang M, Li D, Cai S, Zeng G, Yin Z, Wang B, Li P, Hong X, Chen J, Zou B, Li J
Drug design, development and therapy 2022;16:3197-3213

Curcumin Derivative Cur20 Attenuated Cerebral Ischemic Injury by Antioxidant Effect and HIF-1α/VEGF/TFEB-Activated Angiogenesis.

Zhang R, Zhao T, Zheng B, Zhang Y, Li X, Zhang F, Cen J, Duan S
Frontiers in pharmacology 2021;12:648107

Intraperitoneal injection of Desferal® alleviated the age-related bone loss and senescence of bone marrow stromal cells in rats.

Yi L, Ju Y, He Y, Yin X, Xu Y, Weng T
Stem cell research & therapy 2021 Jan 7;12(1):45

Effects of loading on chondrocyte hypoxia, HIF-1α and VEGF in the mandibular condylar cartilage of young rats.

Yu J, Liang F, Huang H, Pirttiniemi P, Yu D
Orthodontics & craniofacial research 2018 Feb;21(1):41-47

Expression and Correlation of Hypoxia-Inducible Factor-1α (HIF-1α) with Pulmonary Artery Remodeling and Right Ventricular Hypertrophy in Experimental Pulmonary Embolism.

Liu W, Zhang Y, Lu L, Wang L, Chen M, Hu T
Medical science monitor : international medical journal of experimental and clinical research 2017 May 1;23:2083-2088

Anti-inflammatory Effects of Phyllanthus emblica L on Benzopyrene-Induced Precancerous Lung Lesion by Regulating the IL-1β/miR-101/Lin28B Signaling Pathway.

Wang CC, Yuan JR, Wang CF, Yang N, Chen J, Liu D, Song J, Feng L, Tan XB, Jia XB
Integrative cancer therapies 2017 Dec;16(4):505-515

Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways.

Wan J, Wu W
Journal of experimental & clinical cancer research : CR 2016 Jul 26;35(1):119

Expression of BNIP3 and its correlations to hypoxia-induced autophagy and clinicopathological features in salivary adenoid cystic carcinoma.

Chen Z, Wu H, Huang S, Li W, Zhang S, Zheng P, Zhou X, Liu W, Zhang D
Cancer biomarkers : section A of Disease markers 2015;15(4):467-75

HIF‑1 signaling pathway involving iNOS, COX‑2 and caspase‑9 mediates the neuroprotection provided by erythropoietin in the retina of chronic ocular hypertension rats.

Gui D, Li Y, Chen X, Gao D, Yang Y, Li X
Molecular medicine reports 2015 Feb;11(2):1490-6

Semaphorin 4D and hypoxia-inducible factor-1α overexpression is related to prognosis in colorectal carcinoma.

Wang JS, Jing CQ, Shan KS, Chen YZ, Guo XB, Cao ZX, Mu LJ, Peng LP, Zhou ML, Li LP
World journal of gastroenterology 2015 Feb 21;21(7):2191-8

Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1α expression.

Wang T, Zhou YT, Chen XN, Zhu AX
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2014 Sep;47(9):738-45

HIF-1α expression correlates with cellular apoptosis, angiogenesis and clinical prognosis in rectal carcinoma.

Feng L, Tao L, Dawei H, Xuliang L, Xiaodong L
Pathology oncology research : POR 2014 Jul;20(3):603-10

Up-regulation of survivin by AKT and hypoxia-inducible factor 1α contributes to cisplatin resistance in gastric cancer.

Sun XP, Dong X, Lin L, Jiang X, Wei Z, Zhai B, Sun B, Zhang Q, Wang X, Jiang H, Krissansen GW, Qiao H, Sun X
The FEBS journal 2014 Jan;281(1):115-28

Mutations in isocitrate dehydrogenase 2 accelerate glioma cell migration via matrix metalloproteinase-2 and 9.

Fu Y, Zheng Y, Li K, Huang R, Zheng S, An N, Liang A
Biotechnology letters 2012 Mar;34(3):441-6

Improved radiosensitizing effect of the combination of etanidazole and paclitaxel for hepatocellular carcinoma in vivo.

Yang Y, Jin C, Li H, He Y, Liu Z, Bai L, Dou K
Experimental and therapeutic medicine 2012 Feb;3(2):299-303

Puerarin decreases hypoxia inducible factor-1 alpha in the hippocampus of vascular dementia rats.

Wu H, Wang H, Zhang B, Zhang G, Zhang R, Zhang L
Neural regeneration research 2012 Feb 25;7(6):421-5

Effect of erythropoietin on the expression of HIF-1 and iNOS in retina in chronic ocular hypertension rats.

Gui DM, Yang Y, Li X, Gao DW
International journal of ophthalmology 2011;4(1):40-3

Analysis of neovascularization after implanting porous silk fibroin films in rats.

Sun Z, Bai L
Bio-medical materials and engineering 2011;21(4):259-70

Bone marrow mesenchymal stem cells in a three-dimensional gelatin sponge scaffold attenuate inflammation, promote angiogenesis, and reduce cavity formation in experimental spinal cord injury.

Zeng X, Zeng YS, Ma YH, Lu LY, Du BL, Zhang W, Li Y, Chan WY
Cell transplantation 2011;20(11-12):1881-99

Various doses of fractioned irradiation modulates multidrug resistance 1 expression differently through hypoxia-inducible factor 1α in esophageal cancer cells.

Zhang GJ, Gao R, Wang JS, Fu JK, Zhang MX, Jin X
Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus 2011 Sep;24(7):481-8

The role of psychologic stress-induced hypoxia-inducible factor-1α in rat experimental periodontitis.

Huang S, Lu F, Zhang Z, Yang X, Chen Y
Journal of periodontology 2011 Jun;82(6):934-41

Combinational therapy of interferon-α and chemotherapy normalizes tumor vasculature by regulating pericytes including the novel marker RGS5 in melanoma.

Liu P, Zhang C, Chen J, Zhang R, Ren J, Huang Y, Zhu F, Li Z, Wu G
Journal of immunotherapy (Hagerstown, Md. : 1997) 2011 Apr;34(3):320-6

Transplantation of neural stem cells expressing hypoxia-inducible factor-1alpha (HIF-1alpha) improves behavioral recovery in a rat stroke model.

Wu W, Chen X, Hu C, Li J, Yu Z, Cai W
Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia 2010 Jan;17(1):92-5

Antisense hypoxia-inducible factor 1alpha gene therapy enhances the therapeutic efficacy of doxorubicin to combat hepatocellular carcinoma.

Liu F, Wang P, Jiang X, Tan G, Qiao H, Jiang H, Krissansen GW, Sun X
Cancer science 2008 Oct;99(10):2055-61

Correlation between the expression of aquaporin 1 and hypoxia-inducible factor 1 in breast cancer tissues.

Yin T, Yu S, Xiao L, Zhang J, Liu C, Lu Y, Liu C
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2008 Jun;28(3):346-8

Antiangiogenic therapy enhances the efficacy of transcatheter arterial embolization for hepatocellular carcinomas.

Jiang H, Meng Q, Tan H, Pan S, Sun B, Xu R, Sun X
International journal of cancer 2007 Jul 15;121(2):416-24

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Supportive validation

Submitted by: Boster Biological Technology (provider)
Main image
Experimental details: Western blot analysis of HIF-1 alpha/HIF1A using anti-HIF-1 alpha/HIF1A antibody (A00013-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-1 alpha/HIF1A antigen affinity purified polyclonal antibody (Catalog # A00013-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF-1 alpha/HIF1A at approximately 93KD. The expected band size for HIF-1 alpha/HIF1A is at 93KD.
Additional image