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- Antibody Data
- Antigen structure
- References [6]
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- Product number
- NB110-74679 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-74679, RRID:AB_1048939
- Product name
- Rabbit Polyclonal HIF-1 alpha Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. This HIF-1 alpha [Hydroxy Pro564] Antibody is specific for the hydroxylated form of the protein (Pro564). Minimal reactivity occurs with the non-hydroxylated form of the protein.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Simian, Xenopus
- Host
- Rabbit
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Relative Contribution of Prolyl Hydroxylase-Dependent and -Independent Degradation of HIF-1alpha by Proteasomal Pathways in Cerebral Ischemia.
AMP-Activated Protein Kinase α2 in Neutrophils Regulates Vascular Repair via Hypoxia-Inducible Factor-1α and a Network of Proteins Affecting Metabolism and Apoptosis.
Manganese-mediated acceleration of age-related hearing loss in mice.
Rho-kinase inhibition prevents the progression of diabetic nephropathy by downregulating hypoxia-inducible factor 1α.
HEXIM1 down-regulates hypoxia-inducible factor-1α protein stability.
Continuous expression of HIF-1α in neural stem/progenitor cells.
Badawi Y, Shi H
Frontiers in neuroscience 2017;11:239
Frontiers in neuroscience 2017;11:239
AMP-Activated Protein Kinase α2 in Neutrophils Regulates Vascular Repair via Hypoxia-Inducible Factor-1α and a Network of Proteins Affecting Metabolism and Apoptosis.
Abdel Malik R, Zippel N, Frömel T, Heidler J, Zukunft S, Walzog B, Ansari N, Pampaloni F, Wingert S, Rieger MA, Wittig I, Fisslthaler B, Fleming I
Circulation research 2017 Jan 6;120(1):99-109
Circulation research 2017 Jan 6;120(1):99-109
Manganese-mediated acceleration of age-related hearing loss in mice.
Ohgami N, Yajima I, Iida M, Li X, Oshino R, Kumasaka MY, Kato M
Scientific reports 2016 Nov 8;6:36306
Scientific reports 2016 Nov 8;6:36306
Rho-kinase inhibition prevents the progression of diabetic nephropathy by downregulating hypoxia-inducible factor 1α.
Matoba K, Kawanami D, Okada R, Tsukamoto M, Kinoshita J, Ito T, Ishizawa S, Kanazawa Y, Yokota T, Murai N, Matsufuji S, Takahashi-Fujigasaki J, Utsunomiya K
Kidney international 2013 Sep;84(3):545-54
Kidney international 2013 Sep;84(3):545-54
HEXIM1 down-regulates hypoxia-inducible factor-1α protein stability.
Yeh IJ, Ogba N, Bensigner H, Welford SM, Montano MM
The Biochemical journal 2013 Dec 1;456(2):195-204
The Biochemical journal 2013 Dec 1;456(2):195-204
Continuous expression of HIF-1α in neural stem/progenitor cells.
Roitbak T, Surviladze Z, Cunningham LA
Cellular and molecular neurobiology 2011 Jan;31(1):119-33
Cellular and molecular neurobiology 2011 Jan;31(1):119-33
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.
