MA1-16504
antibody from Invitrogen Antibodies
Targeting: HIF1A
bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8
Antibody data
- Antibody Data
- Antigen structure
- References [70]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Other assay [7]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA1-16504 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIF1A Monoclonal Antibody (H1alpha67)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- In Western blot, multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha. For WB, testing on nuclear extracts is recommended. Recommended positive controls: COS-7 nuclear hypoxic induced cell lysate, HeLa hypoxic (CoCl2) cell lysate, HepG2 hypoxic (CoCl2) cell lysate, HeLa hypoxic cell lysate, HepG2 hypoxic cell lysate, HIF-1 alpha Knockout HeLa cell lysate
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Feline, Porcine, Rabbit, Xenopus
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- H1alpha67
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Metabolic Reprogramming and Inflammatory Response Induced by D-Lactate in Bovine Fibroblast-Like Synoviocytes Depends on HIF-1 Activity.
Patient Derived Colonoids as Drug Testing Platforms-Critical Importance of Oxygen Concentration.
Estrogens Regulate Placental Angiogenesis in Horses.
Equine cervical remodeling during placentitis and the prepartum period: a transcriptomic approach.
Compartmentalization of adenosine metabolism in cancer cells and its modulation during acute hypoxia.
HIF1 driven transcriptional activity regulates steroidogenesis and proliferation of bovine granulosa cells.
Molecular Imaging of Extracellular Tumor pH to Reveal Effects of Locoregional Therapy on Liver Cancer Microenvironment.
Targeting LOX-1 Inhibits Colorectal Cancer Metastasis in an Animal Model.
A Nonsense Mitochondrial DNA Mutation Associates with Dysfunction of HIF1α in a Von Hippel-Lindau Renal Oncocytoma.
Hypoxia-induced Rab11-family interacting protein 4 expression promotes migration and invasion of colon cancer and correlates with poor prognosis.
Differential but Complementary HIF1α and HIF2α Transcriptional Regulation.
Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma.
Remote Ischemic Preconditioning Reduces Cerebral Oxidative Stress Following Hypothermic Circulatory Arrest in a Porcine Model.
Hypoxia-induced endothelial-mesenchymal transition is associated with RASAL1 promoter hypermethylation in human coronary endothelial cells.
Prognostic impact of clinicopathological features and expression of biomarkers related to (18)F-FDG uptake in esophageal cancer.
The role of CXC-chemokine receptor CXCR2 and suppressor of cytokine signaling-3 (SOCS-3) in renal cell carcinoma.
The role of aberrant VHL/HIF pathway elements in predicting clinical outcome to pazopanib therapy in patients with metastatic clear-cell renal cell carcinoma.
Analysis of biomarker expression in severe endometriosis and determination of possibilities for targeted intraoperative imaging.
Low-level laser therapy alleviates neuropathic pain and promotes function recovery in rats with chronic constriction injury: possible involvements in hypoxia-inducible factor 1α (HIF-1α).
KAI1 suppresses HIF-1α and VEGF expression by blocking CDCP1-enhanced Src activation in prostate cancer.
Immunohistochemical study of the angiogenetic network of VEGF, HIF1α, VEGFR-2 and endothelial nitric oxide synthase (eNOS) in human breast cancer.
Expression of thioredoxin-1 and hypoxia inducible factor-1α in cerebral arteriovenous malformations: Possible role of redox regulatory factor in neoangiogenic property.
HIF-1α and VEGF are associated with disease progression in esophageal carcinoma.
Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
Astragaloside IV stimulates angiogenesis and increases hypoxia-inducible factor-1α accumulation via phosphatidylinositol 3-kinase/Akt pathway.
Effects of cigarette smoking on hearing recovery from noise-induced temporary hearing threshold shifts in mice.
Evaluation of relationship between HIF-1alpha immunoreactivity and stage, grade, angiogenic profile and proliferative index in bladder urothelial carcinomas.
Vasculogenic mimicry: a new prognostic sign of gastric adenocarcinoma.
Expression of KIT receptor tyrosine kinase in endothelial cells of juvenile brain tumors.
Changes in vascular permeability and expression of different angiogenic factors following anti-angiogenic treatment in rat glioma.
Combinatorial regulation of neuroblastoma tumor progression by N-Myc and hypoxia inducible factor HIF-1alpha.
Enhancing effects of a chromone glycoside, eucryphin, isolated from Astilbe rhizomes on burn wound repair and its mechanism.
Antitumor effects of 2-oxoglutarate through inhibition of angiogenesis in a murine tumor model.
Ethanol and tobacco smoke increase hepatic steatosis and hypoxia in the hypercholesterolemic apoE(-/-) mouse: implications for a "multihit" hypothesis of fatty liver disease.
Ethanol and tobacco smoke increase hepatic steatosis and hypoxia in the hypercholesterolemic apoE(-/-) mouse: implications for a "multihit" hypothesis of fatty liver disease.
Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings.
DNA-dependent protein kinase is involved in heat shock protein-mediated accumulation of hypoxia-inducible factor-1alpha in hypoxic preconditioned HepG2 cells.
Hypoxia causes downregulation of mismatch repair system and genomic instability in stem cells.
Cellular turnover and expression of hypoxic-inducible factor in acute acalculous and calculous cholecystitis.
Endothelial cell KIT expression in human tumours.
Normoxic stabilization of HIF-1alpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk.
Nucleus pulposus cells express HIF-1 alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment.
Akt1 activation can augment hypoxia-inducible factor-1alpha expression by increasing protein translation through a mammalian target of rapamycin-independent pathway.
Understanding hypoxia-induced gene expression in early development: in vitro and in vivo analysis of hypoxia-inducible factor 1-regulated zebra fish insulin-like growth factor binding protein 1 gene expression.
Hypoxia-inducible factor 1 and VEGF upregulate CXCR4 in glioblastoma: implications for angiogenesis and glioma cell invasion.
Axial shortening during distraction osteogenesis leads to enhanced bone formation in a rabbit model through the HIF-1alpha/vascular endothelial growth factor system.
HIF-1alpha-targeted pathways are activated by heat acclimation and contribute to acclimation-ischemic cross-tolerance in the heart.
Expression of hypoxia-inducible factor 1alpha and vascular endothelial growth factor in hepatocellular carcinoma: Impact on neovascularization and survival.
Expression of hypoxia-inducible factor 1alpha and vascular endothelial growth factor in hepatocellular carcinoma: Impact on neovascularization and survival.
Accumulation of hypoxia-inducible factor-1alpha in mouse inner ear by noise stimulation.
Accumulation of hypoxia-inducible factor-1alpha in mouse inner ear by noise stimulation.
HIF-VEGF-VEGFR-2, TNF-alpha and IGF pathways are upregulated in critical human skeletal muscle ischemia as studied with DNA array.
Bimodal effect of hypoxia in cancer: role of hypoxia inducible factor in apoptosis.
Bimodal effect of hypoxia in cancer: role of hypoxia inducible factor in apoptosis.
Prognostic significance of erythropoietin expression in human endometrial carcinoma.
Overexpression of hypoxia-inducible-factor 1alpha(HIF-1alpha) in oesophageal squamous cell carcinoma correlates with lymph node metastasis and pathologic stage.
Hepatic vascular tumors, angiectasis in multiple organs, and impaired spermatogenesis in mice with conditional inactivation of the VHL gene.
Clinical benefit of prostaglandin E1-treatment of patients with ischemic heart disease: stimulation of therapeutic angiogenesis in vital and infarcted myocardium.
Clinical benefit of prostaglandin E1-treatment of patients with ischemic heart disease: stimulation of therapeutic angiogenesis in vital and infarcted myocardium.
HLF/HIF-2alpha is a key factor in retinopathy of prematurity in association with erythropoietin.
Enhanced expression of a novel protein in human cancer cells: a potential aid to cancer diagnosis.
Enhanced expression of a novel protein in human cancer cells: a potential aid to cancer diagnosis.
Enhanced overexpression of an HIF-1/hypoxia-related protein in cancer cells.
Enhanced overexpression of an HIF-1/hypoxia-related protein in cancer cells.
Clinical and experimental evidence of prostaglandin E1-induced angiogenesis in the myocardium of patients with ischemic heart disease.
Clinical and experimental evidence of prostaglandin E1-induced angiogenesis in the myocardium of patients with ischemic heart disease.
Identification of functional hypoxia response elements in the promoter region of the DEC1 and DEC2 genes.
Histone deacetylases induce angiogenesis by negative regulation of tumor suppressor genes.
Molecular regulation of the endothelin-1 gene by hypoxia. Contributions of hypoxia-inducible factor-1, activator protein-1, GATA-2, AND p300/CBP.
Hyperinducibility of hypoxia-responsive genes without p53/p21-dependent checkpoint in aggressive prostate cancer.
Quiroga J, Alarcón P, Manosalva C, Teuber S, Taubert A, Hermosilla C, Hidalgo MA, Carretta MD, Burgos RA
Frontiers in veterinary science 2021;8:625347
Frontiers in veterinary science 2021;8:625347
Patient Derived Colonoids as Drug Testing Platforms-Critical Importance of Oxygen Concentration.
Skovdahl HK, Gopalakrishnan S, Svendsen TD, Granlund AVB, Bakke I, Ginbot ZG, Thorsvik S, Flatberg A, Sporsheim B, Ostrop J, Mollnes TE, Sandvik AK, Bruland T
Frontiers in pharmacology 2021;12:679741
Frontiers in pharmacology 2021;12:679741
Estrogens Regulate Placental Angiogenesis in Horses.
Haneda S, Dini P, Esteller-Vico A, Scoggin KE, Squires EL, Troedsson MH, Daels P, Nambo Y, Ball BA
International journal of molecular sciences 2021 Nov 9;22(22)
International journal of molecular sciences 2021 Nov 9;22(22)
Equine cervical remodeling during placentitis and the prepartum period: a transcriptomic approach.
El-Sheikh Ali H, Scoggin KE, Ruby R, Loynachan A, Boakari Y, Fernandes C, Dini P, Fedorka CE, Loux SC, Esteller-Vico A, Ball BA
Reproduction (Cambridge, England) 2021 May 5;161(6):603-621
Reproduction (Cambridge, England) 2021 May 5;161(6):603-621
Compartmentalization of adenosine metabolism in cancer cells and its modulation during acute hypoxia.
Losenkova K, Zuccarini M, Karikoski M, Laurila J, Boison D, Jalkanen S, Yegutkin GG
Journal of cell science 2020 May 29;133(10)
Journal of cell science 2020 May 29;133(10)
HIF1 driven transcriptional activity regulates steroidogenesis and proliferation of bovine granulosa cells.
Baddela VS, Sharma A, Michaelis M, Vanselow J
Scientific reports 2020 Mar 3;10(1):3906
Scientific reports 2020 Mar 3;10(1):3906
Molecular Imaging of Extracellular Tumor pH to Reveal Effects of Locoregional Therapy on Liver Cancer Microenvironment.
Savic LJ, Schobert IT, Peters D, Walsh JJ, Laage-Gaupp FM, Hamm CA, Tritz N, Doemel LA, Lin M, Sinusas A, Schlachter T, Duncan JS, Hyder F, Coman D, Chapiro J
Clinical cancer research : an official journal of the American Association for Cancer Research 2020 Jan 15;26(2):428-438
Clinical cancer research : an official journal of the American Association for Cancer Research 2020 Jan 15;26(2):428-438
Targeting LOX-1 Inhibits Colorectal Cancer Metastasis in an Animal Model.
Murdocca M, Capuano R, Pucci S, Cicconi R, Polidoro C, Catini A, Martinelli E, Paolesse R, Orlandi A, Mango R, Novelli G, Di Natale C, Sangiuolo F
Frontiers in oncology 2019;9:927
Frontiers in oncology 2019;9:927
A Nonsense Mitochondrial DNA Mutation Associates with Dysfunction of HIF1α in a Von Hippel-Lindau Renal Oncocytoma.
De Luise M, Guarnieri V, Ceccarelli C, D'Agruma L, Porcelli AM, Gasparre G
Oxidative medicine and cellular longevity 2019;2019:8069583
Oxidative medicine and cellular longevity 2019;2019:8069583
Hypoxia-induced Rab11-family interacting protein 4 expression promotes migration and invasion of colon cancer and correlates with poor prognosis.
Wang JZ, Yang SX, Ye F, Xia XP, Shao XX, Xia SL, Zheng B, Xu CL
Molecular medicine reports 2018 Mar;17(3):3797-3806
Molecular medicine reports 2018 Mar;17(3):3797-3806
Differential but Complementary HIF1α and HIF2α Transcriptional Regulation.
Downes NL, Laham-Karam N, Kaikkonen MU, Ylä-Herttuala S
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Jul 5;26(7):1735-1745
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Jul 5;26(7):1735-1745
Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma.
Murakami A, Wang L, Kalhorn S, Schraml P, Rathmell WK, Tan AC, Nemenoff R, Stenmark K, Jiang BH, Reyland ME, Heasley L, Hu CJ
Oncogenesis 2017 Jan 16;6(1):e287
Oncogenesis 2017 Jan 16;6(1):e287
Remote Ischemic Preconditioning Reduces Cerebral Oxidative Stress Following Hypothermic Circulatory Arrest in a Porcine Model.
Arvola O, Haapanen H, Herajärvi J, Anttila T, Puistola U, Karihtala P, Tuominen H, Anttila V, Juvonen T
Seminars in thoracic and cardiovascular surgery 2016 Spring;28(1):92-102
Seminars in thoracic and cardiovascular surgery 2016 Spring;28(1):92-102
Hypoxia-induced endothelial-mesenchymal transition is associated with RASAL1 promoter hypermethylation in human coronary endothelial cells.
Xu X, Tan X, Hulshoff MS, Wilhelmi T, Zeisberg M, Zeisberg EM
FEBS letters 2016 Apr;590(8):1222-33
FEBS letters 2016 Apr;590(8):1222-33
Prognostic impact of clinicopathological features and expression of biomarkers related to (18)F-FDG uptake in esophageal cancer.
Schreurs LM, Smit JK, Pavlov K, Pultrum BB, Pruim J, Groen H, Hollema H, Plukker JT
Annals of surgical oncology 2014 Nov;21(12):3751-7
Annals of surgical oncology 2014 Nov;21(12):3751-7
The role of CXC-chemokine receptor CXCR2 and suppressor of cytokine signaling-3 (SOCS-3) in renal cell carcinoma.
Stofas A, Levidou G, Piperi C, Adamopoulos C, Dalagiorgou G, Bamias A, Karadimou A, Lainakis GA, Papadoukakis S, Stravodimos K, Dimopoulos MA, Patsouris E, Gakiopoulou H, Korkolopoulou P
BMC cancer 2014 Mar 4;14:149
BMC cancer 2014 Mar 4;14:149
The role of aberrant VHL/HIF pathway elements in predicting clinical outcome to pazopanib therapy in patients with metastatic clear-cell renal cell carcinoma.
Choueiri TK, Fay AP, Gagnon R, Lin Y, Bahamon B, Brown V, Rosenberg JE, Hutson TE, Baker-Neblett KL, Carpenter C, Liu Y, Pandite L, Signoretti S
Clinical cancer research : an official journal of the American Association for Cancer Research 2013 Sep 15;19(18):5218-26
Clinical cancer research : an official journal of the American Association for Cancer Research 2013 Sep 15;19(18):5218-26
Analysis of biomarker expression in severe endometriosis and determination of possibilities for targeted intraoperative imaging.
van den Berg LL, Crane LM, van Oosten M, van Dam GM, Simons AH, Hofker HS, Bart J
International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics 2013 Apr;121(1):35-40
International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics 2013 Apr;121(1):35-40
Low-level laser therapy alleviates neuropathic pain and promotes function recovery in rats with chronic constriction injury: possible involvements in hypoxia-inducible factor 1α (HIF-1α).
Hsieh YL, Chou LW, Chang PL, Yang CC, Kao MJ, Hong CZ
The Journal of comparative neurology 2012 Sep 1;520(13):2903-16
The Journal of comparative neurology 2012 Sep 1;520(13):2903-16
KAI1 suppresses HIF-1α and VEGF expression by blocking CDCP1-enhanced Src activation in prostate cancer.
Park JJ, Jin YB, Lee YJ, Lee JS, Lee YS, Ko YG, Lee M
BMC cancer 2012 Mar 6;12:81
BMC cancer 2012 Mar 6;12:81
Immunohistochemical study of the angiogenetic network of VEGF, HIF1α, VEGFR-2 and endothelial nitric oxide synthase (eNOS) in human breast cancer.
Kafousi M, Vrekoussis T, Tsentelierou E, Pavlakis K, Navrozoglou I, Dousias V, Sanidas E, Tsiftsis D, Georgoulias V, Stathopoulos EN
Pathology oncology research : POR 2012 Jan;18(1):33-41
Pathology oncology research : POR 2012 Jan;18(1):33-41
Expression of thioredoxin-1 and hypoxia inducible factor-1α in cerebral arteriovenous malformations: Possible role of redox regulatory factor in neoangiogenic property.
Takagi Y, Kikuta K, Moriwaki T, Aoki T, Nozaki K, Hashimoto N, Miyamoto S
Surgical neurology international 2011;2:61
Surgical neurology international 2011;2:61
HIF-1α and VEGF are associated with disease progression in esophageal carcinoma.
Takala H, Saarnio J, Wiik H, Ohtonen P, Soini Y
The Journal of surgical research 2011 May 1;167(1):41-8
The Journal of surgical research 2011 May 1;167(1):41-8
Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
Nature 2011 Jul 13;475(7355):226-30
Nature 2011 Jul 13;475(7355):226-30
Astragaloside IV stimulates angiogenesis and increases hypoxia-inducible factor-1α accumulation via phosphatidylinositol 3-kinase/Akt pathway.
Zhang L, Liu Q, Lu L, Zhao X, Gao X, Wang Y
The Journal of pharmacology and experimental therapeutics 2011 Aug;338(2):485-91
The Journal of pharmacology and experimental therapeutics 2011 Aug;338(2):485-91
Effects of cigarette smoking on hearing recovery from noise-induced temporary hearing threshold shifts in mice.
Ahn JH, Joo HS, Suh JK, Kim H, So HS, Chung JW
Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology 2011 Aug;32(6):926-32
Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology 2011 Aug;32(6):926-32
Evaluation of relationship between HIF-1alpha immunoreactivity and stage, grade, angiogenic profile and proliferative index in bladder urothelial carcinomas.
Deniz H, Karakök M, Yagci F, Güldür ME
International urology and nephrology 2010 Mar;42(1):103-7
International urology and nephrology 2010 Mar;42(1):103-7
Vasculogenic mimicry: a new prognostic sign of gastric adenocarcinoma.
Li M, Gu Y, Zhang Z, Zhang S, Zhang D, Saleem AF, Zhao X, Sun B
Pathology oncology research : POR 2010 Jun;16(2):259-66
Pathology oncology research : POR 2010 Jun;16(2):259-66
Expression of KIT receptor tyrosine kinase in endothelial cells of juvenile brain tumors.
Puputti M, Tynninen O, Pernilä P, Salmi M, Jalkanen S, Paetau A, Sihto H, Joensuu H
Brain pathology (Zurich, Switzerland) 2010 Jul;20(4):763-70
Brain pathology (Zurich, Switzerland) 2010 Jul;20(4):763-70
Changes in vascular permeability and expression of different angiogenic factors following anti-angiogenic treatment in rat glioma.
Ali MM, Janic B, Babajani-Feremi A, Varma NR, Iskander AS, Anagli J, Arbab AS
PloS one 2010 Jan 15;5(1):e8727
PloS one 2010 Jan 15;5(1):e8727
Combinatorial regulation of neuroblastoma tumor progression by N-Myc and hypoxia inducible factor HIF-1alpha.
Qing G, Skuli N, Mayes PA, Pawel B, Martinez D, Maris JM, Simon MC
Cancer research 2010 Dec 15;70(24):10351-61
Cancer research 2010 Dec 15;70(24):10351-61
Enhancing effects of a chromone glycoside, eucryphin, isolated from Astilbe rhizomes on burn wound repair and its mechanism.
Sumiyoshi M, Kimura Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2010 Aug;17(10):820-9
Phytomedicine : international journal of phytotherapy and phytopharmacology 2010 Aug;17(10):820-9
Antitumor effects of 2-oxoglutarate through inhibition of angiogenesis in a murine tumor model.
Matsumoto K, Obara N, Ema M, Horie M, Naka A, Takahashi S, Imagawa S
Cancer science 2009 Sep;100(9):1639-47
Cancer science 2009 Sep;100(9):1639-47
Ethanol and tobacco smoke increase hepatic steatosis and hypoxia in the hypercholesterolemic apoE(-/-) mouse: implications for a "multihit" hypothesis of fatty liver disease.
Bailey SM, Mantena SK, Millender-Swain T, Cakir Y, Jhala NC, Chhieng D, Pinkerton KE, Ballinger SW
Free radical biology & medicine 2009 Apr 1;46(7):928-38
Free radical biology & medicine 2009 Apr 1;46(7):928-38
Ethanol and tobacco smoke increase hepatic steatosis and hypoxia in the hypercholesterolemic apoE(-/-) mouse: implications for a "multihit" hypothesis of fatty liver disease.
Bailey SM, Mantena SK, Millender-Swain T, Cakir Y, Jhala NC, Chhieng D, Pinkerton KE, Ballinger SW
Free radical biology & medicine 2009 Apr 1;46(7):928-38
Free radical biology & medicine 2009 Apr 1;46(7):928-38
Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings.
Arbab AS, Janic B, Knight RA, Anderson SA, Pawelczyk E, Rad AM, Read EJ, Pandit SD, Frank JA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Sep;22(9):3234-46
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Sep;22(9):3234-46
DNA-dependent protein kinase is involved in heat shock protein-mediated accumulation of hypoxia-inducible factor-1alpha in hypoxic preconditioned HepG2 cells.
Kang MJ, Jung SM, Kim MJ, Bae JH, Kim HB, Kim JY, Park SJ, Song HS, Kim DW, Kang CD, Kim SH
The FEBS journal 2008 Dec;275(23):5969-81
The FEBS journal 2008 Dec;275(23):5969-81
Hypoxia causes downregulation of mismatch repair system and genomic instability in stem cells.
Rodríguez-Jiménez FJ, Moreno-Manzano V, Lucas-Dominguez R, Sánchez-Puelles JM
Stem cells (Dayton, Ohio) 2008 Aug;26(8):2052-62
Stem cells (Dayton, Ohio) 2008 Aug;26(8):2052-62
Cellular turnover and expression of hypoxic-inducible factor in acute acalculous and calculous cholecystitis.
Vakkala M, Laurila JJ, Saarnio J, Koivukangas V, Syrjälä H, Karttunen T, Soini Y, Ala-Kokko TI
Critical care (London, England) 2007;11(5):R116
Critical care (London, England) 2007;11(5):R116
Endothelial cell KIT expression in human tumours.
Sihto H, Tynninen O, Bützow R, Saarialho-Kere U, Joensuu H
The Journal of pathology 2007 Mar;211(4):481-8
The Journal of pathology 2007 Mar;211(4):481-8
Normoxic stabilization of HIF-1alpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk.
Agrawal A, Guttapalli A, Narayan S, Albert TJ, Shapiro IM, Risbud MV
American journal of physiology. Cell physiology 2007 Aug;293(2):C621-31
American journal of physiology. Cell physiology 2007 Aug;293(2):C621-31
Nucleus pulposus cells express HIF-1 alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment.
Risbud MV, Guttapalli A, Stokes DG, Hawkins D, Danielson KG, Schaer TP, Albert TJ, Shapiro IM
Journal of cellular biochemistry 2006 May 1;98(1):152-9
Journal of cellular biochemistry 2006 May 1;98(1):152-9
Akt1 activation can augment hypoxia-inducible factor-1alpha expression by increasing protein translation through a mammalian target of rapamycin-independent pathway.
Pore N, Jiang Z, Shu HK, Bernhard E, Kao GD, Maity A
Molecular cancer research : MCR 2006 Jul;4(7):471-9
Molecular cancer research : MCR 2006 Jul;4(7):471-9
Understanding hypoxia-induced gene expression in early development: in vitro and in vivo analysis of hypoxia-inducible factor 1-regulated zebra fish insulin-like growth factor binding protein 1 gene expression.
Kajimura S, Aida K, Duan C
Molecular and cellular biology 2006 Feb;26(3):1142-55
Molecular and cellular biology 2006 Feb;26(3):1142-55
Hypoxia-inducible factor 1 and VEGF upregulate CXCR4 in glioblastoma: implications for angiogenesis and glioma cell invasion.
Zagzag D, Lukyanov Y, Lan L, Ali MA, Esencay M, Mendez O, Yee H, Voura EB, Newcomb EW
Laboratory investigation; a journal of technical methods and pathology 2006 Dec;86(12):1221-32
Laboratory investigation; a journal of technical methods and pathology 2006 Dec;86(12):1221-32
Axial shortening during distraction osteogenesis leads to enhanced bone formation in a rabbit model through the HIF-1alpha/vascular endothelial growth factor system.
Mori S, Akagi M, Kikuyama A, Yasuda Y, Hamanishi C
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2006 Apr;24(4):653-63
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2006 Apr;24(4):653-63
HIF-1alpha-targeted pathways are activated by heat acclimation and contribute to acclimation-ischemic cross-tolerance in the heart.
Maloyan A, Eli-Berchoer L, Semenza GL, Gerstenblith G, Stern MD, Horowitz M
Physiological genomics 2005 Sep 21;23(1):79-88
Physiological genomics 2005 Sep 21;23(1):79-88
Expression of hypoxia-inducible factor 1alpha and vascular endothelial growth factor in hepatocellular carcinoma: Impact on neovascularization and survival.
Huang GW, Yang LY, Lu WQ
World journal of gastroenterology 2005 Mar 21;11(11):1705-8
World journal of gastroenterology 2005 Mar 21;11(11):1705-8
Expression of hypoxia-inducible factor 1alpha and vascular endothelial growth factor in hepatocellular carcinoma: Impact on neovascularization and survival.
Huang GW, Yang LY, Lu WQ
World journal of gastroenterology 2005 Mar 21;11(11):1705-8
World journal of gastroenterology 2005 Mar 21;11(11):1705-8
Accumulation of hypoxia-inducible factor-1alpha in mouse inner ear by noise stimulation.
Chung JW, Kang HH, Shin JE, Kim JU
Neuroreport 2004 Oct 25;15(15):2353-6
Neuroreport 2004 Oct 25;15(15):2353-6
Accumulation of hypoxia-inducible factor-1alpha in mouse inner ear by noise stimulation.
Chung JW, Kang HH, Shin JE, Kim JU
Neuroreport 2004 Oct 25;15(15):2353-6
Neuroreport 2004 Oct 25;15(15):2353-6
HIF-VEGF-VEGFR-2, TNF-alpha and IGF pathways are upregulated in critical human skeletal muscle ischemia as studied with DNA array.
Tuomisto TT, Rissanen TT, Vajanto I, Korkeela A, Rutanen J, Ylä-Herttuala S
Atherosclerosis 2004 May;174(1):111-20
Atherosclerosis 2004 May;174(1):111-20
Bimodal effect of hypoxia in cancer: role of hypoxia inducible factor in apoptosis.
Wang Y, Pakunlu RI, Tsao W, Pozharov V, Minko T
Molecular pharmaceutics 2004 Mar-Apr;1(2):156-65
Molecular pharmaceutics 2004 Mar-Apr;1(2):156-65
Bimodal effect of hypoxia in cancer: role of hypoxia inducible factor in apoptosis.
Wang Y, Pakunlu RI, Tsao W, Pozharov V, Minko T
Molecular pharmaceutics 2004 Mar-Apr;1(2):156-65
Molecular pharmaceutics 2004 Mar-Apr;1(2):156-65
Prognostic significance of erythropoietin expression in human endometrial carcinoma.
Acs G, Xu X, Chu C, Acs P, Verma A
Cancer 2004 Jun 1;100(11):2376-86
Cancer 2004 Jun 1;100(11):2376-86
Overexpression of hypoxia-inducible-factor 1alpha(HIF-1alpha) in oesophageal squamous cell carcinoma correlates with lymph node metastasis and pathologic stage.
Kurokawa T, Miyamoto M, Kato K, Cho Y, Kawarada Y, Hida Y, Shinohara T, Itoh T, Okushiba S, Kondo S, Katoh H
British journal of cancer 2003 Sep 15;89(6):1042-7
British journal of cancer 2003 Sep 15;89(6):1042-7
Hepatic vascular tumors, angiectasis in multiple organs, and impaired spermatogenesis in mice with conditional inactivation of the VHL gene.
Ma W, Tessarollo L, Hong SB, Baba M, Southon E, Back TC, Spence S, Lobe CG, Sharma N, Maher GW, Pack S, Vortmeyer AO, Guo C, Zbar B, Schmidt LS
Cancer research 2003 Sep 1;63(17):5320-8
Cancer research 2003 Sep 1;63(17):5320-8
Clinical benefit of prostaglandin E1-treatment of patients with ischemic heart disease: stimulation of therapeutic angiogenesis in vital and infarcted myocardium.
Mehrabi MR, Serbecic N, Tamaddon F, Pacher R, Horvath R, Mall G, Glogar HD
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2003 May-Jun;57(3-4):173-8
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2003 May-Jun;57(3-4):173-8
Clinical benefit of prostaglandin E1-treatment of patients with ischemic heart disease: stimulation of therapeutic angiogenesis in vital and infarcted myocardium.
Mehrabi MR, Serbecic N, Tamaddon F, Pacher R, Horvath R, Mall G, Glogar HD
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2003 May-Jun;57(3-4):173-8
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2003 May-Jun;57(3-4):173-8
HLF/HIF-2alpha is a key factor in retinopathy of prematurity in association with erythropoietin.
Morita M, Ohneda O, Yamashita T, Takahashi S, Suzuki N, Nakajima O, Kawauchi S, Ema M, Shibahara S, Udono T, Tomita K, Tamai M, Sogawa K, Yamamoto M, Fujii-Kuriyama Y
The EMBO journal 2003 Mar 3;22(5):1134-46
The EMBO journal 2003 Mar 3;22(5):1134-46
Enhanced expression of a novel protein in human cancer cells: a potential aid to cancer diagnosis.
Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M
Cell biology and toxicology 2002;18(2):87-96
Cell biology and toxicology 2002;18(2):87-96
Enhanced expression of a novel protein in human cancer cells: a potential aid to cancer diagnosis.
Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M
Cell biology and toxicology 2002;18(2):87-96
Cell biology and toxicology 2002;18(2):87-96
Enhanced overexpression of an HIF-1/hypoxia-related protein in cancer cells.
Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M
Environmental health perspectives 2002 Oct;110 Suppl 5(Suppl 5):783-8
Environmental health perspectives 2002 Oct;110 Suppl 5(Suppl 5):783-8
Enhanced overexpression of an HIF-1/hypoxia-related protein in cancer cells.
Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M
Environmental health perspectives 2002 Oct;110 Suppl 5:783-8
Environmental health perspectives 2002 Oct;110 Suppl 5:783-8
Clinical and experimental evidence of prostaglandin E1-induced angiogenesis in the myocardium of patients with ischemic heart disease.
Mehrabi MR, Serbecic N, Tamaddon F, Kaun C, Huber K, Pacher R, Wild T, Mall G, Wojta J, Glogar HD
Cardiovascular research 2002 Nov;56(2):214-24
Cardiovascular research 2002 Nov;56(2):214-24
Clinical and experimental evidence of prostaglandin E1-induced angiogenesis in the myocardium of patients with ischemic heart disease.
Mehrabi MR, Serbecic N, Tamaddon F, Kaun C, Huber K, Pacher R, Wild T, Mall G, Wojta J, Glogar HD
Cardiovascular research 2002 Nov;56(2):214-24
Cardiovascular research 2002 Nov;56(2):214-24
Identification of functional hypoxia response elements in the promoter region of the DEC1 and DEC2 genes.
Miyazaki K, Kawamoto T, Tanimoto K, Nishiyama M, Honda H, Kato Y
The Journal of biological chemistry 2002 Dec 6;277(49):47014-21
The Journal of biological chemistry 2002 Dec 6;277(49):47014-21
Histone deacetylases induce angiogenesis by negative regulation of tumor suppressor genes.
Kim MS, Kwon HJ, Lee YM, Baek JH, Jang JE, Lee SW, Moon EJ, Kim HS, Lee SK, Chung HY, Kim CW, Kim KW
Nature medicine 2001 Apr;7(4):437-43
Nature medicine 2001 Apr;7(4):437-43
Molecular regulation of the endothelin-1 gene by hypoxia. Contributions of hypoxia-inducible factor-1, activator protein-1, GATA-2, AND p300/CBP.
Yamashita K, Discher DJ, Hu J, Bishopric NH, Webster KA
The Journal of biological chemistry 2001 Apr 20;276(16):12645-53
The Journal of biological chemistry 2001 Apr 20;276(16):12645-53
Hyperinducibility of hypoxia-responsive genes without p53/p21-dependent checkpoint in aggressive prostate cancer.
Salnikow K, Costa M, Figg WD, Blagosklonny MV
Cancer research 2000 Oct 15;60(20):5630-4
Cancer research 2000 Oct 15;60(20):5630-4
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- Western Blot analysis of 50 µg cobalt chloride (CoCl2) induced COS-7 nuclear extracts.
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- Western blot analysis of HIF1A in 50 µg cobalt chloride induced COS-7 nuclear extracts. Samples were incubated in HIF1A monoclonal antibody (Product # MA1-16504.
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- Knockdown validation by Immunocytochemistry analysis of HIF1A in immersion fixed DFO treated Hela cells (left) and HIF-1 knockout HeLa cells (right). Samples were incubated in HIF1A monoclonal antibody (Product # MA1-16504) using a dilution of followed by a NorthernLights™ 557-conjugated Donkey Anti-Mouse IgG secondary antibody. Cells were counterstained with DAPI (blue). Specific staining was localized to nuclei.
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- Immunocytochemistry analysis of HIF1A in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFOA. Samples were incubated in HIF1A monoclonal antibody (Product # MA1-16504) using a dilution of 1 µg/mL followed by donkey anti-rabbit secondary antibody and counterstained with DAPI (blue).
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- Knockdown validation by Immunocytochemistry analysis of HIF1A in immersion fixed DFO treated Hela cells (left) and HIF-1 knockout HeLa cells (right). Samples were incubated in HIF1A monoclonal antibody (Product # MA1-16504) using a dilution of followed by a NorthernLights™ 557-conjugated Donkey Anti-Mouse IgG secondary antibody. Cells were counterstained with DAPI (blue). Specific staining was localized to nuclei.
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- Immunohistochemical analysis of HIF1A in human kidney. Samples were incubated in HIF1A monoclonal antibody (Product # MA1-16504). Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.
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- Immunohistochemical analysis of HIF1A in human glioblastoma multiforme. Samples were incubated in HIF1A monoclonal antibody (Product # MA1-16504). Antibody conjugated to biotin.
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- Figure 6 D-Lactate induces HIF-1alpha protein accumulation under normoxic conditions in bFLS. bFLS were stimulated with 5 mM D-lactate for 6 h under normoxic (20% O 2 ) and hypoxic (1% O 2 ) conditions. bTNF-alpha was used as the positive control. HIF-1alpha stabilization was detected in total protein extracts by Western blot using cobalt chloride (CoCl 2 ) as the hypoxia-mimetic agent. (A) Representative HIF-1alpha immunoblot is shown. (B) Densitometry HIF-1alpha values were quantified using Image Studio Lite v5.2 software and normalized to beta-actin. Each bar represents the mean +- SEM, n = 3. ** p < 0.01; **** p < 0.0001; n.s ., not significant.
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- FIGURE 1 Effects of oxygen concentration on growth, cell-death, gene expression and metabolism in human colonoids (A) . Experimental design. Differentiated colonoids from six donors were either grown continuously at 20% O 2 or calibrated at 2% O 2 for 16h before treated with TNF (100ngml -1 ), IL17 (100ngml -1 ) or a combination (TNF/IL17) for 24h (B) Detection of morphology and cell death by live imaging of untreated colonoids. Left image shows a 3D depth encoded image of one colonoid at 20% O 2 , according to the color scale from red (superficial) to blue (deep) on the right side of the image. Center and right image panels show colonoids in 20% or 2% O 2 respectively, where NucBlue (r) Live reagent (Hoechst 33342) stains all cell nuclei (blue) and propidium iodide stains dead cell nuclei (red). Scale bars 100um (C) Expression of epithelial cell markers as measured by flow cytometry in untreated colonoids from three donors, in 20% O 2 or 2% O 2 as depicted in panel (A) ( n = 3 + 3 for each marker). Relative change between oxygen concentrations normalized to 20% O 2 (red line at y = 1) displayed, with mean and individual normalized values (D) and (E) Immunostaining of differentiated colonoids grown at 20% or 2% O 2 , representative selection from five assays. Scale bars 50um. (D) Ki67 positive nuclei stained brown, counterstaining with hematoxylin. The graph shows percentage of Ki67 positive nuclei in four independent experiments indicated by dots. Bars show mean with SD (E)
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- Figure 4 Immunohistochemical evaluation of letrozole-treated chorioallantoises (CA). ( A ) Immunohistochemical demonstration of capillary densities in chorioallantoic membranes of letrozole-treated and control mares using vWF. The capillary density tended to be greater in the control CAs than in the letrozole-treated CAs, p = 0.07; ( B ) A strong cytoplasmic signal for VEGF is noted within the chorionic epithelium of letrozole-treated group. No difference was observed in the abundance of HIF1A + cells between the groups. ( B ) Red boxes (letrozole) and blue circles (control) represent values for the capillary density scores in the chorioallantoic samples.
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- Figure 1 Expression analysis of HIF1A in granulosa cells. ( a ) indicates the mRNA expression of HIF1A under different FSH and IGF1 concentrations. ( b ) Indicates the detection of HIF1A protein under normoxia. The lane numbers 1-4 indicate the western runs of individual protein lysates. Columns 1 and 2 represent duplicates of HIF1A probing in FSH (20 ng/mL) and IGF1(50 ng/mL) treated GC under normoxia while columns 3 and 4 represent the Beta actin (BACT) probing in the corresponding samples. The arrow marks in ( b ) indicate the HIF1A (columns 1 and 2) and BACT (columns 3 and 4) protein bands. The image is obtained with the exposure time of 8 seconds in Simple Western instrument. ( c ) illustrates the immunolocalization of HIF1A in different growing ovarian follicles of abattoir ovaries. The brown color immunochemical signal indicates the HIF1A staining inside the follicle. 400x optical magnification was used for primary and secondary and large antral follicle's wall. 100x optical magnification was used for tertiary follicles in the pictures. Inside dotted box indicates the specific follicle for primary, secondary and tertiary follicle sections whereas dotted box points the GC layer in large antral follicle's wall. Data were presented in Mean +- SEM values of three independent cell culture experiments (n = 3) for Fig. 1a. Significant differences were acknowledged at the minimum level of p < 0.05 by one way repeated measures analysis of variance. Pairwise comparisons were ana
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- Figure 8 Chromatin immunoprecipitation analysis. ( a ) shows the nucleotide sequence between the physical location of 59122186 to 59122473 nucleotides on chromosome 10 in the bovine genome. Manually curated TATA box and the putative HIF1 binding sites were highlighted in blue and yellow colors. The primer binding sites were indicated with an underline. ( b ) adopted JASPAR Sequence logo of the HIF1 binding site based on ChIP seq data in humans. ( c ) indicates the agarose gel pictures of immune-precipitated and input DNA samples. ( d , e ) depict the relative quantification of HIF1 binding events on the CYP19A1 promoter based on percent input and fold change methods, respectively under FSH and IGF1 Supplemented conditions. Data were represented in mean +- SEM values of three independent experiments (n = 3). Significant differences were acknowledged at the minimum level of p < 0.05 by one way repeated measures analysis of variance. Pairwise comparisons were analyzed using Post hoc Tuckey test.