MA1-16511

Product number: MA1-16511 - Provider product page
Provider: Invitrogen Antibodies
Product name: HIF1A Monoclonal Antibody (H1alpha67)
Antibody type: Monoclonal
Antigen: Other
Description: In Western blot, multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha. For WB, testing on nuclear extracts is recommended.
Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Porcine, Rabbit
Host: Mouse
Isotype: IgG
Antibody clone number: H1alpha67
Vial size: 100 µL
Concentration: 1.0 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

HIF1A protein structure - MA1-16511 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 LCN2-HIF1A pathway regulates EDN1 expression in human HSCs. ( a ) Representative IF images of co-localization LCN2 receptor ( SLC22A17 ) and alpha-SMA in AH livers. Hoechst 33,258 (blue) was used to label nuclei, TSA-Cy5 visualized SLC22A17 (red) and TSA-Cy3 visualized alpha-SMA (green). Overlay of SLC22A17 and alpha-SMA showed co-expression (yellow). ( b ) LX-2 cells were exposed to 50 uM human recombinant LCN2. ACTA2 , COL1A1 , AGT and EDN1 gene expression was measured by qPCR (n = 3). ( c ) Intracellular free calcium concentration of LX-2 cells was detected using Fura-2 fluorescence. ( d ) Human primary HSCs transduced with adenovirus-mediated overexpression of GFP or LCN2. ACTA2 , COL1A1 , AGT and EDN1 gene expression were measured by qPCR (n = 3). ( e ) Human primary HSCs transduced with adenovirus-mediated overexpression of GFP or LCN2. HIF1A expression was detected by Western Blot (n = 3) and the original blots are presented in Supplementary Figure 5 . ( f ) HIF1A siRNA was transfected into human primary HSCs and EDN1 gene expression measured by qPCR (n = 3). ( g ) ChIP-qPCR was used to detect binding affinity of HIF1A to the EDN1 gene promoter in human primary HSCs (n = 3). * p < 0.05 by two-tailed Student's t test. Scale bar: 100 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 PTBP3 increases HIF-1alpha protein levels in colon cancer cells. a-b Western blot of HIF-1alpha expression in HCT116 and SW480 cells +- PTBP3 OE grown under normoxia or hypoxia (1% O 2 ) for 4 h. GAPDH was used as a loading control. c-d, Western blot of HIF-1alpha expression in HCT116 and SW480 cells +- shRNA PTBP3 KD grown under normoxia or hypoxia (1% O 2 ) for 4 h. GAPDH was used as a loading control. e Western blot of HIF-1alpha expression in tumor xenografts formed by HCT116 cells +- PTBP3 KD/OE. GAPDH was used as a loading control. f Representative examples of IHC-based correlation between PTBP3 and HIF-1alpha expression in colorectal tumor sections from TMAs. g Fisher's exact test analysis of the correlation of PTBP3 and HIF-1alpha expression in CRC TMAs. h Relative HRE-Luc activity in HCT116 cells +- PTBP3 OE was detected by the Dual Luciferase Reporter Assay System. The Rluc activity was used for normalization. Data are presented as the means +- SD for experiments in triplicate. *** p < 0.001