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LearnMA1-16518
antibody from Invitrogen Antibodies
Targeting: HIF1A
bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- MA1-16518 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIF1A Monoclonal Antibody (ESEE122)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Suggested positive control: Cos7 CoCl2-treated nuclear extract.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- ESEE122
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Losartan improves the therapeutic effect of metronomic cyclophosphamide in triple negative mammary cancer models.
Mainetti LE, Rico MJ, Kaufman CD, Grillo MC, Guercetti J, Baglioni MV, Del Giúdice A, Capitani MC, Fusini M, Rozados VR, Scharovsky OG
Oncotarget 2020 Aug 11;11(32):3048-3060
Oncotarget 2020 Aug 11;11(32):3048-3060
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HIF1A in 0.5 mg/mL Hypoxic HeLa lysate. Samples were incubated in HIF1A MA1-16518 antibody (Product # MA1-16518). This experiment was performed under reducing conditions using the 12-230 kDa separation system..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of HIF1A in a cell cytospin from a lavage of a murine skin pouch infected with S. aureus. Samples were incubated in HIF1A monoclonal antibody (Product # MA1-16518). HIF-1 alpha (red dye). 100X magnification. Blue: DAPI nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of HIF1A in a cell cytospin from a lavage of a murine skin pouch infected with S. aureus. Samples were incubated in HIF1A monoclonal antibody (Product # MA1-16518). HIF-1 alpha (red dye). 100X magnification. Blue: DAPI nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HIF1A in hypoxia-induced human placenta. Samples were incubated in HIF1A monoclonal antibody (Product # MA1-16518).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HIF1A in formalin-fixed paraffin-embedded tissue section of human renal cancer xenograft. Samples were incubated in HIF1A monoclonal antibody (Product # MA1-16518) using a dilution of 1:200 dilution. The antibody generated a strong cytoplasmic staining mainly in the cancer cells. Only a fraction of cells depicted nuclear staining, while weak to negligible positivity was seen in the tumor stromal cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HIF1A in human placenta (from sea level; 4 µM paraffin-embedded section). Samples were incubated in HIF1A monoclonal antibody (Product # MA1-16518 using a dilution of 1:100.
