MA1-46457

antibody from Invitrogen Antibodies

Targeting: HIF1A bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8

Provider product page for MA1-46457

Western blot

ELISA

Immunohistochemistry

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [44]
Comments [0]
Validations
Western blot [3]
Other assay [1]

Submit

Product number: MA1-46457 - Provider product page
Provider: Invitrogen Antibodies
Product name: HIF1A Monoclonal Antibody (H1alpha67), Biotin
Antibody type: Monoclonal
Antigen: Other
Description: In Western blot, multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha. For WB, testing on nuclear extracts is recommended.
Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Porcine, Rabbit
Host: Mouse
Conjugate: Biotin
Isotype: IgG
Antibody clone number: H1alpha67
Vial size: 100 µL
Concentration: 0.99 mg/mL
Storage: 4° C, store in dark

HIF1A protein structure - MA1-46457 shown in red.

Submitted references The role of CXC-chemokine receptor CXCR2 and suppressor of cytokine signaling-3 (SOCS-3) in renal cell carcinoma.

Stofas A, Levidou G, Piperi C, Adamopoulos C, Dalagiorgou G, Bamias A, Karadimou A, Lainakis GA, Papadoukakis S, Stravodimos K, Dimopoulos MA, Patsouris E, Gakiopoulou H, Korkolopoulou P
BMC cancer 2014 Mar 4;14:149

The role of aberrant VHL/HIF pathway elements in predicting clinical outcome to pazopanib therapy in patients with metastatic clear-cell renal cell carcinoma.

Choueiri TK, Fay AP, Gagnon R, Lin Y, Bahamon B, Brown V, Rosenberg JE, Hutson TE, Baker-Neblett KL, Carpenter C, Liu Y, Pandite L, Signoretti S
Clinical cancer research : an official journal of the American Association for Cancer Research 2013 Sep 15;19(18):5218-26

Analysis of biomarker expression in severe endometriosis and determination of possibilities for targeted intraoperative imaging.

van den Berg LL, Crane LM, van Oosten M, van Dam GM, Simons AH, Hofker HS, Bart J
International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics 2013 Apr;121(1):35-40

Low-level laser therapy alleviates neuropathic pain and promotes function recovery in rats with chronic constriction injury: possible involvements in hypoxia-inducible factor 1α (HIF-1α).

Hsieh YL, Chou LW, Chang PL, Yang CC, Kao MJ, Hong CZ
The Journal of comparative neurology 2012 Sep 1;520(13):2903-16

KAI1 suppresses HIF-1α and VEGF expression by blocking CDCP1-enhanced Src activation in prostate cancer.

Park JJ, Jin YB, Lee YJ, Lee JS, Lee YS, Ko YG, Lee M
BMC cancer 2012 Mar 6;12:81

Immunohistochemical study of the angiogenetic network of VEGF, HIF1α, VEGFR-2 and endothelial nitric oxide synthase (eNOS) in human breast cancer.

Kafousi M, Vrekoussis T, Tsentelierou E, Pavlakis K, Navrozoglou I, Dousias V, Sanidas E, Tsiftsis D, Georgoulias V, Stathopoulos EN
Pathology oncology research : POR 2012 Jan;18(1):33-41

Expression of thioredoxin-1 and hypoxia inducible factor-1α in cerebral arteriovenous malformations: Possible role of redox regulatory factor in neoangiogenic property.

Takagi Y, Kikuta K, Moriwaki T, Aoki T, Nozaki K, Hashimoto N, Miyamoto S
Surgical neurology international 2011;2:61

Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.

Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
Nature 2011 Jul 13;475(7355):226-30

Astragaloside IV stimulates angiogenesis and increases hypoxia-inducible factor-1α accumulation via phosphatidylinositol 3-kinase/Akt pathway.

Zhang L, Liu Q, Lu L, Zhao X, Gao X, Wang Y
The Journal of pharmacology and experimental therapeutics 2011 Aug;338(2):485-91

Effects of cigarette smoking on hearing recovery from noise-induced temporary hearing threshold shifts in mice.

Ahn JH, Joo HS, Suh JK, Kim H, So HS, Chung JW
Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology 2011 Aug;32(6):926-32

Evaluation of relationship between HIF-1alpha immunoreactivity and stage, grade, angiogenic profile and proliferative index in bladder urothelial carcinomas.

Deniz H, Karakök M, Yagci F, Güldür ME
International urology and nephrology 2010 Mar;42(1):103-7

Vasculogenic mimicry: a new prognostic sign of gastric adenocarcinoma.

Li M, Gu Y, Zhang Z, Zhang S, Zhang D, Saleem AF, Zhao X, Sun B
Pathology oncology research : POR 2010 Jun;16(2):259-66

Expression of KIT receptor tyrosine kinase in endothelial cells of juvenile brain tumors.

Puputti M, Tynninen O, Pernilä P, Salmi M, Jalkanen S, Paetau A, Sihto H, Joensuu H
Brain pathology (Zurich, Switzerland) 2010 Jul;20(4):763-70

Changes in vascular permeability and expression of different angiogenic factors following anti-angiogenic treatment in rat glioma.

Ali MM, Janic B, Babajani-Feremi A, Varma NR, Iskander AS, Anagli J, Arbab AS
PloS one 2010 Jan 15;5(1):e8727

Combinatorial regulation of neuroblastoma tumor progression by N-Myc and hypoxia inducible factor HIF-1alpha.

Qing G, Skuli N, Mayes PA, Pawel B, Martinez D, Maris JM, Simon MC
Cancer research 2010 Dec 15;70(24):10351-61

Enhancing effects of a chromone glycoside, eucryphin, isolated from Astilbe rhizomes on burn wound repair and its mechanism.

Sumiyoshi M, Kimura Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2010 Aug;17(10):820-9

Antitumor effects of 2-oxoglutarate through inhibition of angiogenesis in a murine tumor model.

Matsumoto K, Obara N, Ema M, Horie M, Naka A, Takahashi S, Imagawa S
Cancer science 2009 Sep;100(9):1639-47

Ethanol and tobacco smoke increase hepatic steatosis and hypoxia in the hypercholesterolemic apoE(-/-) mouse: implications for a "multihit" hypothesis of fatty liver disease.

Bailey SM, Mantena SK, Millender-Swain T, Cakir Y, Jhala NC, Chhieng D, Pinkerton KE, Ballinger SW
Free radical biology & medicine 2009 Apr 1;46(7):928-38

Ethanol and tobacco smoke increase hepatic steatosis and hypoxia in the hypercholesterolemic apoE(-/-) mouse: implications for a "multihit" hypothesis of fatty liver disease.

Bailey SM, Mantena SK, Millender-Swain T, Cakir Y, Jhala NC, Chhieng D, Pinkerton KE, Ballinger SW
Free radical biology & medicine 2009 Apr 1;46(7):928-38

Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings.

Arbab AS, Janic B, Knight RA, Anderson SA, Pawelczyk E, Rad AM, Read EJ, Pandit SD, Frank JA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Sep;22(9):3234-46

Cellular turnover and expression of hypoxic-inducible factor in acute acalculous and calculous cholecystitis.

Vakkala M, Laurila JJ, Saarnio J, Koivukangas V, Syrjälä H, Karttunen T, Soini Y, Ala-Kokko TI
Critical care (London, England) 2007;11(5):R116

Endothelial cell KIT expression in human tumours.

Sihto H, Tynninen O, Bützow R, Saarialho-Kere U, Joensuu H
The Journal of pathology 2007 Mar;211(4):481-8

Normoxic stabilization of HIF-1alpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk.

Agrawal A, Guttapalli A, Narayan S, Albert TJ, Shapiro IM, Risbud MV
American journal of physiology. Cell physiology 2007 Aug;293(2):C621-31

Nucleus pulposus cells express HIF-1 alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment.

Risbud MV, Guttapalli A, Stokes DG, Hawkins D, Danielson KG, Schaer TP, Albert TJ, Shapiro IM
Journal of cellular biochemistry 2006 May 1;98(1):152-9

Akt1 activation can augment hypoxia-inducible factor-1alpha expression by increasing protein translation through a mammalian target of rapamycin-independent pathway.

Pore N, Jiang Z, Shu HK, Bernhard E, Kao GD, Maity A
Molecular cancer research : MCR 2006 Jul;4(7):471-9

Hypoxia-inducible factor 1 and VEGF upregulate CXCR4 in glioblastoma: implications for angiogenesis and glioma cell invasion.

Zagzag D, Lukyanov Y, Lan L, Ali MA, Esencay M, Mendez O, Yee H, Voura EB, Newcomb EW
Laboratory investigation; a journal of technical methods and pathology 2006 Dec;86(12):1221-32

Axial shortening during distraction osteogenesis leads to enhanced bone formation in a rabbit model through the HIF-1alpha/vascular endothelial growth factor system.

Mori S, Akagi M, Kikuyama A, Yasuda Y, Hamanishi C
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2006 Apr;24(4):653-63

Expression of hypoxia-inducible factor 1alpha and vascular endothelial growth factor in hepatocellular carcinoma: Impact on neovascularization and survival.

Huang GW, Yang LY, Lu WQ
World journal of gastroenterology 2005 Mar 21;11(11):1705-8

Expression of hypoxia-inducible factor 1alpha and vascular endothelial growth factor in hepatocellular carcinoma: Impact on neovascularization and survival.

Huang GW, Yang LY, Lu WQ
World journal of gastroenterology 2005 Mar 21;11(11):1705-8

Accumulation of hypoxia-inducible factor-1alpha in mouse inner ear by noise stimulation.

Chung JW, Kang HH, Shin JE, Kim JU
Neuroreport 2004 Oct 25;15(15):2353-6

Accumulation of hypoxia-inducible factor-1alpha in mouse inner ear by noise stimulation.

Chung JW, Kang HH, Shin JE, Kim JU
Neuroreport 2004 Oct 25;15(15):2353-6

HIF-VEGF-VEGFR-2, TNF-alpha and IGF pathways are upregulated in critical human skeletal muscle ischemia as studied with DNA array.

Tuomisto TT, Rissanen TT, Vajanto I, Korkeela A, Rutanen J, Ylä-Herttuala S
Atherosclerosis 2004 May;174(1):111-20

Bimodal effect of hypoxia in cancer: role of hypoxia inducible factor in apoptosis.

Wang Y, Pakunlu RI, Tsao W, Pozharov V, Minko T
Molecular pharmaceutics 2004 Mar-Apr;1(2):156-65

Bimodal effect of hypoxia in cancer: role of hypoxia inducible factor in apoptosis.

Wang Y, Pakunlu RI, Tsao W, Pozharov V, Minko T
Molecular pharmaceutics 2004 Mar-Apr;1(2):156-65

Prognostic significance of erythropoietin expression in human endometrial carcinoma.

Acs G, Xu X, Chu C, Acs P, Verma A
Cancer 2004 Jun 1;100(11):2376-86

Overexpression of hypoxia-inducible-factor 1alpha(HIF-1alpha) in oesophageal squamous cell carcinoma correlates with lymph node metastasis and pathologic stage.

Kurokawa T, Miyamoto M, Kato K, Cho Y, Kawarada Y, Hida Y, Shinohara T, Itoh T, Okushiba S, Kondo S, Katoh H
British journal of cancer 2003 Sep 15;89(6):1042-7

Hepatic vascular tumors, angiectasis in multiple organs, and impaired spermatogenesis in mice with conditional inactivation of the VHL gene.

Ma W, Tessarollo L, Hong SB, Baba M, Southon E, Back TC, Spence S, Lobe CG, Sharma N, Maher GW, Pack S, Vortmeyer AO, Guo C, Zbar B, Schmidt LS
Cancer research 2003 Sep 1;63(17):5320-8

HLF/HIF-2alpha is a key factor in retinopathy of prematurity in association with erythropoietin.

Morita M, Ohneda O, Yamashita T, Takahashi S, Suzuki N, Nakajima O, Kawauchi S, Ema M, Shibahara S, Udono T, Tomita K, Tamai M, Sogawa K, Yamamoto M, Fujii-Kuriyama Y
The EMBO journal 2003 Mar 3;22(5):1134-46

Enhanced expression of a novel protein in human cancer cells: a potential aid to cancer diagnosis.

Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M
Cell biology and toxicology 2002;18(2):87-96

Enhanced expression of a novel protein in human cancer cells: a potential aid to cancer diagnosis.

Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M
Cell biology and toxicology 2002;18(2):87-96

Enhanced overexpression of an HIF-1/hypoxia-related protein in cancer cells.

Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M
Environmental health perspectives 2002 Oct;110 Suppl 5(Suppl 5):783-8

Enhanced overexpression of an HIF-1/hypoxia-related protein in cancer cells.

Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M
Environmental health perspectives 2002 Oct;110 Suppl 5:783-8

Histone deacetylases induce angiogenesis by negative regulation of tumor suppressor genes.

Kim MS, Kwon HJ, Lee YM, Baek JH, Jang JE, Lee SW, Moon EJ, Kim HS, Lee SK, Chung HY, Kim CW, Kim KW
Nature medicine 2001 Apr;7(4):437-43

Molecular regulation of the endothelin-1 gene by hypoxia. Contributions of hypoxia-inducible factor-1, activator protein-1, GATA-2, AND p300/CBP.

Yamashita K, Discher DJ, Hu J, Bishopric NH, Webster KA
The Journal of biological chemistry 2001 Apr 20;276(16):12645-53

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: On day 1, MEF cells (+/+, -/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100 µg of protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution. The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts using Product # MA1-46457.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HIF1A in cobalt chloride treated/untreated COS-7 nuclear extracts. Sample was incubated in HIF1A monoclonal antibody (Product # MA1-46457).
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Expression of thioredoxin-1 (Trx-1) and hypoxia-inducible factor-1alpha (HIF-1alpha) in cerebral arteriovenous malformations. (a) Trx-1 immunoreactivity was elevated in endothelial cells and perivascular tissue in arteriovenous malformations. (b) Venous component of arteriovenous malformations. Trx-1 immunoreactivity was elevated in endothelial cells (c) Arterial component of arteriovenous malformations. Trx-1 immunoreactivity was also elevated in endothelial cells. (d) Normal small vein in perinidal area. Trx-1 immunoreactivity was hardly detected. (e) Normal small artery in perinidal area. Trx-1 immunoreactivity was also hardly detected. (f) HIF-1alpha immunoreactivity was elevated in endothelial cells and perivascular tissue in arteriovenous malformations. (g) Arterial component of arteriovenous malformations. HIF-1alpha immunoreactivity was elevated in endothelial cells (h) Venous component of arteriovenous malformations. HIF-1alpha immunoreactivity was also elevated in endothelial cells. (i) Normal small vein in perinidal area. HIF-1alpha immunoreactivity was hardly detected. (j) Normal small artery in perinidal area. HIF-1alpha immunoreactivity was also hardly detected
Conjugate: Biotin