PA1-16627

antibody from Invitrogen Antibodies

Targeting: HIF1A bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8

Western blot

Gel shift

Antibody data

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Validation data

Product number: PA1-16627 - Provider product page
Provider: Invitrogen Antibodies
Product name: HIF1A Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: This antibody has 93% sequence homology with bovine and 90% sequence homology with mouse. Suggested positive control: Cos-7 Hypoxia induced nuclear extract, COS-7 nuclear extracts (treated).
Reactivity: Human, Mouse, Rat, Bovine, Porcine
Host: Rabbit
Isotype: IgG
Vial size: 100 μL
Concentration: 1 mg/mL
Storage: -20°C, Avoid Freeze/Thaw Cycles

HIF1A protein structure - PA1-16627 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of HIF1A was performed using 70% confluent log phase HeLa cells treated with 150µM of CoCl2 for 48 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HIF1A Rabbit Polyclonal Antibody(Product # PA1-16627) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows untreated cells with cytoplasmic signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of HIF1A was performed using 70% confluent log phase HeLa cells treated with 150µM of CoCl2 for 48 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HIF1A Rabbit Polyclonal Antibody(Product # PA1-16627) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows untreated cells with cytoplasmic signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.