Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [61]
- Comments [0]
- Validations
- Western blot [15]
- Immunocytochemistry [4]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX127309 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX127309, RRID:AB_2616089
- Product name
- HIF1 alpha antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
Submitted references Chloramphenicol Induces Autophagy and Inhibits the Hypoxia Inducible Factor-1 Alpha Pathway in Non-Small Cell Lung Cancer Cells.
Significant antitumor effect of an antibody against TMEM180, a new colorectal cancer-specific molecule.
Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses.
Autosomal dominant retinitis pigmentosa-associated gene PRPF8 is essential for hypoxia-induced mitophagy through regulating ULK1 mRNA splicing.
Acute intermittent hypoxia and rehabilitative training following cervical spinal injury alters neuronal hypoxia- and plasticity-associated protein expression.
Suppression of COUP-TFII upregulates angiogenin and promotes angiogenesis in endometriosis.
Monocarboxylate transporter-1 promotes osteoblast differentiation via suppression of p53, a negative regulator of osteoblast differentiation.
TMEM207 hinders the tumour suppressor function of WWOX in oral squamous cell carcinoma.
Changes in cell fate determine the regenerative and functional capacity of the developing kidney before and after release of obstruction.
Hypoxic stress upregulates Kir2.1 expression by a pathway including hypoxic-inducible factor-1α and dynamin2 in brain capillary endothelial cells.
Oxaloacetate induces apoptosis in HepG2 cells via inhibition of glycolysis.
Autophagy‑mediated adaptation of hepatocellular carcinoma cells to hypoxia‑mimicking conditions constitutes an attractive therapeutic target.
Glycine Is a Predictor for a Suppressive Effect of Fucoxanthinol on Colonosphere Formation Under Hypoxia.
Improvement of islet function and survival by integration of perfluorodecalin into microcapsules in vivo and in vitro.
Development of a computational promoter with highly efficient expression in tumors.
AHNAK2 is a Novel Prognostic Marker and Oncogenic Protein for Clear Cell Renal Cell Carcinoma.
Astaxanthin prevents ischemia-reperfusion injury of the steatotic liver in mice.
Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.
An Adaptation System to Avoid Apoptosis via Autophagy Under Hypoxic Conditions in Pancreatic Cancer Cells.
Hypoxia favors myosin heavy chain beta gene expression in an Hif-1alpha-dependent manner.
ANGPTL1 Interacts with Integrin α1β1 to Suppress HCC Angiogenesis and Metastasis by Inhibiting JAK2/STAT3 Signaling.
Adenylate kinase hCINAP determines self-renewal of colorectal cancer stem cells by facilitating LDHA phosphorylation.
Involvement of Notch1 signaling in malignant progression of A549 cells subjected to prolonged cadmium exposure.
Hypoxia-inducible factor-1α plays roles in Epstein-Barr virus's natural life cycle and tumorigenesis by inducing lytic infection through direct binding to the immediate-early BZLF1 gene promoter.
Effect of increasing body condition on oxidative stress and mitochondrial biogenesis in subcutaneous adipose tissue depot of nonlactating dairy cows.
Long noncoding RNA LncHIFCAR/MIR31HG is a HIF-1α co-activator driving oral cancer progression.
Endothelial SCUBE2 Interacts With VEGFR2 and Regulates VEGF-Induced Angiogenesis.
Loss of smooth muscle cell hypoxia inducible factor-1α underlies increased vascular contractility in pulmonary hypertension.
Antitumor Activity of the Chinese Medicine JC-001 Is Mediated by Immunomodulation in a Murine Model of Hepatocellular Carcinoma.
Hypoxia-inducible factor-1α promotes cell survival during ammonia stress response in ovarian cancer stem-like cells.
Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
Interaction between von Hippel-Lindau Protein and Fatty Acid Synthase Modulates Hypoxia Target Gene Expression.
Protective Effect of Caffeic Acid Derivatives on tert-Butyl Hydroperoxide-Induced Oxidative Hepato-Toxicity and Mitochondrial Dysfunction in HepG2 Cells.
microRNA-183 Mediates Protective Postconditioning of the Liver by Repressing Apaf-1.
MiR-182 promotes proliferation and invasion and elevates the HIF-1α-VEGF-A axis in breast cancer cells by targeting FBXW7.
HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells.
Combination of ciclopirox olamine and sphingosine-1-phosphate as granulation enhancer in diabetic wounds.
Foxp3 enhances HIF-1α target gene expression in human bladder cancer through decreasing its ubiquitin-proteasomal degradation.
Melatonin promotes cardiomyogenesis of embryonic stem cells via inhibition of HIF-1α stabilization.
Regulation of HIF1α under Hypoxia by APE1/Ref-1 Impacts CA9 Expression: Dual Targeting in Patient-Derived 3D Pancreatic Cancer Models.
Mitochondrial Lon is over-expressed in high-grade gliomas, and mediates hypoxic adaptation: potential role of Lon as a therapeutic target in glioma.
Simvastatin prevents and reverses chronic pulmonary hypertension in newborn rats via pleiotropic inhibition of RhoA signaling.
Role of Mitochondrial DNA Copy Number Alteration in Human Renal Cell Carcinoma.
Inhibition of hypoxia inducible factor-1α attenuates abdominal aortic aneurysm progression through the down-regulation of matrix metalloproteinases.
A novel family of fluorescent hypoxia sensors reveal strong heterogeneity in tumor hypoxia at the cellular level.
Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Lysosomal recruitment of TSC2 is a universal response to cellular stress.
Suppressive Effects of Glucose-Dependent Insulinotropic Polypeptide on Cardiac Hypertrophy and Fibrosis in Angiotensin II-Infused Mouse Models.
Efficient long-term survival of cell grafts after myocardial infarction with thick viable cardiac tissue entirely from pluripotent stem cells.
Enhancement of vascular endothelial growth factor release in long-term drug-treated breast cancer via transient receptor potential channel 5-Ca(2+)-hypoxia-inducible factor 1α pathway.
Increased Serine-Arginine (SR) Protein Phosphorylation Changes Pre-mRNA Splicing in Hypoxia.
Cytoprotective effects of fisetin against hypoxia-induced cell death in PC12 cells.
Diffuse-type gastric cancer cells switch their driver pathways from FGFR2 signaling to SDF1/CXCR4 axis in hypoxic tumor microenvironments.
YC-1 inhibits proliferation of breast cancer cells by down-regulating EZH2 expression via activation of c-Cbl and ERK.
PlGF mediates neutrophil elastase-induced airway epithelial cell apoptosis and emphysema.
PTEN regulates plasma membrane expression of glucose transporter 1 and glucose uptake in thyroid cancer cells.
RAGE mediates S100A4-induced cell motility via MAPK/ERK and hypoxia signaling and is a prognostic biomarker for human colorectal cancer metastasis.
Wnt signaling directs a metabolic program of glycolysis and angiogenesis in colon cancer.
The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression.
Quantitative proteomic analysis of sphere-forming stem-like oral cancer cells.
Matrix metalloproteinase (MMP)-1 induces lung alveolar epithelial cell migration and proliferation, protects from apoptosis, and represses mitochondrial oxygen consumption.
Hsu HL, Liao PL, Cheng YW, Huang SH, Wu CH, Li CH, Kang JJ
International journal of molecular sciences 2019 Jan 3;20(1)
International journal of molecular sciences 2019 Jan 3;20(1)
Significant antitumor effect of an antibody against TMEM180, a new colorectal cancer-specific molecule.
Yasunaga M, Saijou S, Hanaoka S, Anzai T, Tsumura R, Matsumura Y
Cancer science 2019 Feb;110(2):761-770
Cancer science 2019 Feb;110(2):761-770
Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses.
Kurelac I, Iommarini L, Vatrinet R, Amato LB, De Luise M, Leone G, Girolimetti G, Umesh Ganesh N, Bridgeman VL, Ombrato L, Columbaro M, Ragazzi M, Gibellini L, Sollazzo M, Feichtinger RG, Vidali S, Baldassarre M, Foriel S, Vidone M, Cossarizza A, Grifoni D, Kofler B, Malanchi I, Porcelli AM, Gasparre G
Nature communications 2019 Feb 22;10(1):903
Nature communications 2019 Feb 22;10(1):903
Autosomal dominant retinitis pigmentosa-associated gene PRPF8 is essential for hypoxia-induced mitophagy through regulating ULK1 mRNA splicing.
Xu G, Li T, Chen J, Li C, Zhao H, Yao C, Dong H, Wen K, Wang K, Zhao J, Xia Q, Zhou T, Zhang H, Gao P, Li A, Pan X
Autophagy 2018;14(10):1818-1830
Autophagy 2018;14(10):1818-1830
Acute intermittent hypoxia and rehabilitative training following cervical spinal injury alters neuronal hypoxia- and plasticity-associated protein expression.
Hassan A, Arnold BM, Caine S, Toosi BM, Verge VMK, Muir GD
PloS one 2018;13(5):e0197486
PloS one 2018;13(5):e0197486
Suppression of COUP-TFII upregulates angiogenin and promotes angiogenesis in endometriosis.
Fu JL, Hsiao KY, Lee HC, Li WN, Chang N, Wu MH, Tsai SJ
Human reproduction (Oxford, England) 2018 Jul 3;
Human reproduction (Oxford, England) 2018 Jul 3;
Monocarboxylate transporter-1 promotes osteoblast differentiation via suppression of p53, a negative regulator of osteoblast differentiation.
Sasa K, Yoshimura K, Yamada A, Suzuki D, Miyamoto Y, Imai H, Nagayama K, Maki K, Yamamoto M, Kamijo R
Scientific reports 2018 Jul 12;8(1):10579
Scientific reports 2018 Jul 12;8(1):10579
TMEM207 hinders the tumour suppressor function of WWOX in oral squamous cell carcinoma.
Bunai K, Okubo H, Hano K, Inoue K, Kito Y, Saigo C, Shibata T, Takeuchi T
Journal of cellular and molecular medicine 2018 Feb;22(2):1026-1033
Journal of cellular and molecular medicine 2018 Feb;22(2):1026-1033
Changes in cell fate determine the regenerative and functional capacity of the developing kidney before and after release of obstruction.
Nagalakshmi VK, Li M, Shah S, Gigliotti JC, Klibanov AL, Epstein FH, Chevalier RL, Gomez RA, Sequeira-Lopez MLS
Clinical science (London, England : 1979) 2018 Dec 12;132(23):2519-2545
Clinical science (London, England : 1979) 2018 Dec 12;132(23):2519-2545
Hypoxic stress upregulates Kir2.1 expression by a pathway including hypoxic-inducible factor-1α and dynamin2 in brain capillary endothelial cells.
Yamamura H, Suzuki Y, Yamamura H, Asai K, Giles W, Imaizumi Y
American journal of physiology. Cell physiology 2018 Aug 1;315(2):C202-C213
American journal of physiology. Cell physiology 2018 Aug 1;315(2):C202-C213
Oxaloacetate induces apoptosis in HepG2 cells via inhibition of glycolysis.
Kuang Y, Han X, Xu M, Yang Q
Cancer medicine 2018 Apr;7(4):1416-1429
Cancer medicine 2018 Apr;7(4):1416-1429
Autophagy‑mediated adaptation of hepatocellular carcinoma cells to hypoxia‑mimicking conditions constitutes an attractive therapeutic target.
Owada S, Endo H, Shida Y, Okada C, Ito K, Nezu T, Tatemichi M
Oncology reports 2018 Apr;39(4):1805-1812
Oncology reports 2018 Apr;39(4):1805-1812
Glycine Is a Predictor for a Suppressive Effect of Fucoxanthinol on Colonosphere Formation Under Hypoxia.
Terasaki M, Ogawa Y, Endo T, Maeda H, Hamada J, Osada K, Miyashita K, Mutoh M
Anticancer research 2018 Apr;38(4):2169-2179
Anticancer research 2018 Apr;38(4):2169-2179
Improvement of islet function and survival by integration of perfluorodecalin into microcapsules in vivo and in vitro.
Lee SH, Park HS, Yang Y, Lee EY, Kim JW, Khang G, Yoon KH
Journal of tissue engineering and regenerative medicine 2018 Apr;12(4):e2110-e2122
Journal of tissue engineering and regenerative medicine 2018 Apr;12(4):e2110-e2122
Development of a computational promoter with highly efficient expression in tumors.
Ho SY, Chang BH, Chung CH, Lin YL, Chuang CH, Hsieh PJ, Huang WC, Tsai NM, Huang SC, Liu YK, Lo YC, Liao KW
BMC cancer 2018 Apr 27;18(1):480
BMC cancer 2018 Apr 27;18(1):480
AHNAK2 is a Novel Prognostic Marker and Oncogenic Protein for Clear Cell Renal Cell Carcinoma.
Wang M, Li X, Zhang J, Yang Q, Chen W, Jin W, Huang YR, Yang R, Gao WQ
Theranostics 2017;7(5):1100-1113
Theranostics 2017;7(5):1100-1113
Astaxanthin prevents ischemia-reperfusion injury of the steatotic liver in mice.
Li S, Takahara T, Fujino M, Fukuhara Y, Sugiyama T, Li XK, Takahara S
PloS one 2017;12(11):e0187810
PloS one 2017;12(11):e0187810
Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.
Zera K, Zastre J
PloS one 2017;12(10):e0186707
PloS one 2017;12(10):e0186707
An Adaptation System to Avoid Apoptosis via Autophagy Under Hypoxic Conditions in Pancreatic Cancer Cells.
Owada S, Ito K, Endo H, Shida Y, Okada C, Nezu T, Tatemichi M
Anticancer research 2017 Sep;37(9):4927-4934
Anticancer research 2017 Sep;37(9):4927-4934
Hypoxia favors myosin heavy chain beta gene expression in an Hif-1alpha-dependent manner.
Binó L, Procházková J, Radaszkiewicz KA, Kučera J, Kudová J, Pacherník J, Kubala L
Oncotarget 2017 Oct 13;8(48):83684-83697
Oncotarget 2017 Oct 13;8(48):83684-83697
ANGPTL1 Interacts with Integrin α1β1 to Suppress HCC Angiogenesis and Metastasis by Inhibiting JAK2/STAT3 Signaling.
Yan Q, Jiang L, Liu M, Yu D, Zhang Y, Li Y, Fang S, Li Y, Zhu YH, Yuan YF, Guan XY
Cancer research 2017 Nov 1;77(21):5831-5845
Cancer research 2017 Nov 1;77(21):5831-5845
Adenylate kinase hCINAP determines self-renewal of colorectal cancer stem cells by facilitating LDHA phosphorylation.
Ji Y, Yang C, Tang Z, Yang Y, Tian Y, Yao H, Zhu X, Zhang Z, Ji J, Zheng X
Nature communications 2017 May 18;8:15308
Nature communications 2017 May 18;8:15308
Involvement of Notch1 signaling in malignant progression of A549 cells subjected to prolonged cadmium exposure.
Fujiki K, Inamura H, Miyayama T, Matsuoka M
The Journal of biological chemistry 2017 May 12;292(19):7942-7953
The Journal of biological chemistry 2017 May 12;292(19):7942-7953
Hypoxia-inducible factor-1α plays roles in Epstein-Barr virus's natural life cycle and tumorigenesis by inducing lytic infection through direct binding to the immediate-early BZLF1 gene promoter.
Kraus RJ, Yu X, Cordes BA, Sathiamoorthi S, Iempridee T, Nawandar DM, Ma S, Romero-Masters JC, McChesney KG, Lin Z, Makielski KR, Lee DL, Lambert PF, Johannsen EC, Kenney SC, Mertz JE
PLoS pathogens 2017 Jun;13(6):e1006404
PLoS pathogens 2017 Jun;13(6):e1006404
Effect of increasing body condition on oxidative stress and mitochondrial biogenesis in subcutaneous adipose tissue depot of nonlactating dairy cows.
Laubenthal L, Ruda L, Sultana N, Winkler J, Rehage J, Meyer U, Dänicke S, Sauerwein H, Häussler S
Journal of dairy science 2017 Jun;100(6):4976-4986
Journal of dairy science 2017 Jun;100(6):4976-4986
Long noncoding RNA LncHIFCAR/MIR31HG is a HIF-1α co-activator driving oral cancer progression.
Shih JW, Chiang WF, Wu ATH, Wu MH, Wang LY, Yu YL, Hung YW, Wang WC, Chu CY, Hung CL, Changou CA, Yen Y, Kung HJ
Nature communications 2017 Jun 22;8:15874
Nature communications 2017 Jun 22;8:15874
Endothelial SCUBE2 Interacts With VEGFR2 and Regulates VEGF-Induced Angiogenesis.
Lin YC, Chao TY, Yeh CT, Roffler SR, Kannagi R, Yang RB
Arteriosclerosis, thrombosis, and vascular biology 2017 Jan;37(1):144-155
Arteriosclerosis, thrombosis, and vascular biology 2017 Jan;37(1):144-155
Loss of smooth muscle cell hypoxia inducible factor-1α underlies increased vascular contractility in pulmonary hypertension.
Barnes EA, Chen CH, Sedan O, Cornfield DN
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Feb;31(2):650-662
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Feb;31(2):650-662
Antitumor Activity of the Chinese Medicine JC-001 Is Mediated by Immunomodulation in a Murine Model of Hepatocellular Carcinoma.
Chuang MH, Chang JT, Hsu LJ, Jan MS, Lu FJ
Integrative cancer therapies 2017 Dec;16(4):516-525
Integrative cancer therapies 2017 Dec;16(4):516-525
Hypoxia-inducible factor-1α promotes cell survival during ammonia stress response in ovarian cancer stem-like cells.
Kitajima S, Lee KL, Hikasa H, Sun W, Huang RY, Yang H, Matsunaga S, Yamaguchi T, Araki M, Kato H, Poellinger L
Oncotarget 2017 Dec 29;8(70):114481-114494
Oncotarget 2017 Dec 29;8(70):114481-114494
Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
Wang LT, Chiou SS, Chai CY, Hsi E, Chiang CM, Huang SK, Wang SN, Yokoyama KK, Hsu SH
Oncogene 2017 Aug;36(31):4405-4414
Oncogene 2017 Aug;36(31):4405-4414
Interaction between von Hippel-Lindau Protein and Fatty Acid Synthase Modulates Hypoxia Target Gene Expression.
Sun W, Kato H, Kitajima S, Lee KL, Gradin K, Okamoto T, Poellinger L
Scientific reports 2017 Aug 3;7(1):7190
Scientific reports 2017 Aug 3;7(1):7190
Protective Effect of Caffeic Acid Derivatives on tert-Butyl Hydroperoxide-Induced Oxidative Hepato-Toxicity and Mitochondrial Dysfunction in HepG2 Cells.
Tsai TH, Yu CH, Chang YP, Lin YT, Huang CJ, Kuo YH, Tsai PJ
Molecules (Basel, Switzerland) 2017 Apr 28;22(5)
Molecules (Basel, Switzerland) 2017 Apr 28;22(5)
microRNA-183 Mediates Protective Postconditioning of the Liver by Repressing Apaf-1.
Lin HC, Liu SY, Yen EY, Li TK, Lai IR
Antioxidants & redox signaling 2017 Apr 10;26(11):583-597
Antioxidants & redox signaling 2017 Apr 10;26(11):583-597
MiR-182 promotes proliferation and invasion and elevates the HIF-1α-VEGF-A axis in breast cancer cells by targeting FBXW7.
Chiang CH, Chu PY, Hou MF, Hung WC
American journal of cancer research 2016;6(8):1785-98
American journal of cancer research 2016;6(8):1785-98
HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells.
Kudová J, Procházková J, Vašiček O, Perečko T, Sedláčková M, Pešl M, Pacherník J, Kubala L
PloS one 2016;11(6):e0158358
PloS one 2016;11(6):e0158358
Combination of ciclopirox olamine and sphingosine-1-phosphate as granulation enhancer in diabetic wounds.
Lim NS, Sham A, Chee SM, Chan C, Raghunath M
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 2016 Sep;24(5):795-809
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 2016 Sep;24(5):795-809
Foxp3 enhances HIF-1α target gene expression in human bladder cancer through decreasing its ubiquitin-proteasomal degradation.
Jou YC, Tsai YS, Lin CT, Tung CL, Shen CH, Tsai HT, Yang WH, Chang HI, Chen SY, Tzai TS
Oncotarget 2016 Oct 4;7(40):65403-65417
Oncotarget 2016 Oct 4;7(40):65403-65417
Melatonin promotes cardiomyogenesis of embryonic stem cells via inhibition of HIF-1α stabilization.
Kudová J, Vašíček O, Číž M, Kubala L
Journal of pineal research 2016 Nov;61(4):493-503
Journal of pineal research 2016 Nov;61(4):493-503
Regulation of HIF1α under Hypoxia by APE1/Ref-1 Impacts CA9 Expression: Dual Targeting in Patient-Derived 3D Pancreatic Cancer Models.
Logsdon DP, Grimard M, Luo M, Shahda S, Jiang Y, Tong Y, Yu Z, Zyromski N, Schipani E, Carta F, Supuran CT, Korc M, Ivan M, Kelley MR, Fishel ML
Molecular cancer therapeutics 2016 Nov;15(11):2722-2732
Molecular cancer therapeutics 2016 Nov;15(11):2722-2732
Mitochondrial Lon is over-expressed in high-grade gliomas, and mediates hypoxic adaptation: potential role of Lon as a therapeutic target in glioma.
Di K, Lomeli N, Wood SD, Vanderwal CD, Bota DA
Oncotarget 2016 Nov 22;7(47):77457-77467
Oncotarget 2016 Nov 22;7(47):77457-77467
Simvastatin prevents and reverses chronic pulmonary hypertension in newborn rats via pleiotropic inhibition of RhoA signaling.
Wong MJ, Kantores C, Ivanovska J, Jain A, Jankov RP
American journal of physiology. Lung cellular and molecular physiology 2016 Nov 1;311(5):L985-L999
American journal of physiology. Lung cellular and molecular physiology 2016 Nov 1;311(5):L985-L999
Role of Mitochondrial DNA Copy Number Alteration in Human Renal Cell Carcinoma.
Lin CS, Lee HT, Lee MH, Pan SC, Ke CY, Chiu AW, Wei YH
International journal of molecular sciences 2016 May 25;17(6)
International journal of molecular sciences 2016 May 25;17(6)
Inhibition of hypoxia inducible factor-1α attenuates abdominal aortic aneurysm progression through the down-regulation of matrix metalloproteinases.
Tsai SH, Huang PH, Hsu YJ, Peng YJ, Lee CH, Wang JC, Chen JW, Lin SJ
Scientific reports 2016 Jul 1;6:28612
Scientific reports 2016 Jul 1;6:28612
A novel family of fluorescent hypoxia sensors reveal strong heterogeneity in tumor hypoxia at the cellular level.
Erapaneedi R, Belousov VV, Schäfers M, Kiefer F
The EMBO journal 2016 Jan 4;35(1):102-13
The EMBO journal 2016 Jan 4;35(1):102-13
Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Xu YM, Wu DD, Zheng W, Yu FY, Yang F, Yao Y, Zhou Y, Ching YP, Lau AT
Oncotarget 2016 Feb 2;7(5):6146-58
Oncotarget 2016 Feb 2;7(5):6146-58
Lysosomal recruitment of TSC2 is a universal response to cellular stress.
Demetriades C, Plescher M, Teleman AA
Nature communications 2016 Feb 12;7:10662
Nature communications 2016 Feb 12;7:10662
Suppressive Effects of Glucose-Dependent Insulinotropic Polypeptide on Cardiac Hypertrophy and Fibrosis in Angiotensin II-Infused Mouse Models.
Hiromura M, Mori Y, Kohashi K, Terasaki M, Shinmura K, Negoro T, Kawashima H, Kogure M, Wachi T, Watanabe R, Sato K, Kushima H, Tomoyasu M, Nakano Y, Yamada Y, Watanabe T, Hirano T
Circulation journal : official journal of the Japanese Circulation Society 2016 Aug 25;80(9):1988-97
Circulation journal : official journal of the Japanese Circulation Society 2016 Aug 25;80(9):1988-97
Efficient long-term survival of cell grafts after myocardial infarction with thick viable cardiac tissue entirely from pluripotent stem cells.
Matsuo T, Masumoto H, Tajima S, Ikuno T, Katayama S, Minakata K, Ikeda T, Yamamizu K, Tabata Y, Sakata R, Yamashita JK
Scientific reports 2015 Nov 20;5:16842
Scientific reports 2015 Nov 20;5:16842
Enhancement of vascular endothelial growth factor release in long-term drug-treated breast cancer via transient receptor potential channel 5-Ca(2+)-hypoxia-inducible factor 1α pathway.
Zhu Y, Pan Q, Meng H, Jiang Y, Mao A, Wang T, Hua D, Yao X, Jin J, Ma X
Pharmacological research 2015 Mar;93:36-42
Pharmacological research 2015 Mar;93:36-42
Increased Serine-Arginine (SR) Protein Phosphorylation Changes Pre-mRNA Splicing in Hypoxia.
Jakubauskiene E, Vilys L, Makino Y, Poellinger L, Kanopka A
The Journal of biological chemistry 2015 Jul 17;290(29):18079-89
The Journal of biological chemistry 2015 Jul 17;290(29):18079-89
Cytoprotective effects of fisetin against hypoxia-induced cell death in PC12 cells.
Chen PY, Ho YR, Wu MJ, Huang SP, Chen PK, Tai MH, Ho CT, Yen JH
Food & function 2015 Jan;6(1):287-96
Food & function 2015 Jan;6(1):287-96
Diffuse-type gastric cancer cells switch their driver pathways from FGFR2 signaling to SDF1/CXCR4 axis in hypoxic tumor microenvironments.
Kinoshita H, Yashiro M, Fukuoka T, Hasegawa T, Morisaki T, Kasashima H, Masuda G, Noda S, Hirakawa K
Carcinogenesis 2015 Dec;36(12):1511-20
Carcinogenesis 2015 Dec;36(12):1511-20
YC-1 inhibits proliferation of breast cancer cells by down-regulating EZH2 expression via activation of c-Cbl and ERK.
Chang LC, Lin HY, Tsai MT, Chou RH, Lee FY, Teng CM, Hsieh MT, Hung HY, Huang LJ, Yu YL, Kuo SC
British journal of pharmacology 2014 Sep;171(17):4010-25
British journal of pharmacology 2014 Sep;171(17):4010-25
PlGF mediates neutrophil elastase-induced airway epithelial cell apoptosis and emphysema.
Hou HH, Cheng SL, Chung KP, Wei SC, Tsao PN, Lu HH, Wang HC, Yu CJ
Respiratory research 2014 Sep 5;15:106
Respiratory research 2014 Sep 5;15:106
PTEN regulates plasma membrane expression of glucose transporter 1 and glucose uptake in thyroid cancer cells.
Morani F, Phadngam S, Follo C, Titone R, Aimaretti G, Galetto A, Alabiso O, Isidoro C
Journal of molecular endocrinology 2014 Oct;53(2):247-58
Journal of molecular endocrinology 2014 Oct;53(2):247-58
RAGE mediates S100A4-induced cell motility via MAPK/ERK and hypoxia signaling and is a prognostic biomarker for human colorectal cancer metastasis.
Dahlmann M, Okhrimenko A, Marcinkowski P, Osterland M, Herrmann P, Smith J, Heizmann CW, Schlag PM, Stein U
Oncotarget 2014 May 30;5(10):3220-33
Oncotarget 2014 May 30;5(10):3220-33
Wnt signaling directs a metabolic program of glycolysis and angiogenesis in colon cancer.
Pate KT, Stringari C, Sprowl-Tanio S, Wang K, TeSlaa T, Hoverter NP, McQuade MM, Garner C, Digman MA, Teitell MA, Edwards RA, Gratton E, Waterman ML
The EMBO journal 2014 Jul 1;33(13):1454-73
The EMBO journal 2014 Jul 1;33(13):1454-73
The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression.
Chakravarthi BV, Pathi SS, Goswami MT, Cieślik M, Zheng H, Nallasivam S, Arekapudi SR, Jing X, Siddiqui J, Athanikar J, Carskadon SL, Lonigro RJ, Kunju LP, Chinnaiyan AM, Palanisamy N, Varambally S
Oncotarget 2014 Aug 30;5(16):6654-69
Oncotarget 2014 Aug 30;5(16):6654-69
Quantitative proteomic analysis of sphere-forming stem-like oral cancer cells.
Misuno K, Liu X, Feng S, Hu S
Stem cell research & therapy 2013;4(6):156
Stem cell research & therapy 2013;4(6):156
Matrix metalloproteinase (MMP)-1 induces lung alveolar epithelial cell migration and proliferation, protects from apoptosis, and represses mitochondrial oxygen consumption.
Herrera I, Cisneros J, Maldonado M, Ramírez R, Ortiz-Quintero B, Anso E, Chandel NS, Selman M, Pardo A
The Journal of biological chemistry 2013 Sep 6;288(36):25964-75
The Journal of biological chemistry 2013 Sep 6;288(36):25964-75
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HCT116 mock B: HCT116 treated with 150uM CoCl2 C: HCT116 treated with 300uM CoCl2 GTX127309 diluted at 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HIF1 alpha antibody detects HIF1 alpha protein in CoCl2-treated samples by Wesern blot analysis. hypoxia condition by HeLa lysate with 500?M CoCl2 treatment at 1:1000 dilution.A: 30 µg of HeLa cell lysate (normoxia)B: 30 µg of HeLa cell lysate (500?M CoCl2 treatment for 24 hrs)7.5% SDS-PAGEHIF1 alpha antibody (GTX127309) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HIF1 alpha antibody detects HIF1 alpha protein in CoCl2-treated samples by Western blot analysis.A. 30 µg NIH-3T3 whole cell lysate/extract (untreated)B. 30 µg NIH-3T3 whole cell lysate/extract (200µM CoCl2 treatment for 24hr)HIF1 alpha antibody (GTX127309) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HIF1 alpha antibody detects HIF1 alpha protein by Western blot analysis.A. 30 µg HeLa whole cell lysate/extract (untreated)B. 30 µg HeLa whole cell lysate/extract (500uM CoCl2 treatment for 24hr)7.5 % SDS-PAGEHIF1 alpha antibody (GTX127309) dilution: 1:5000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HIF1 alpha antibody detects HIF1 alpha protein in 1% O2-treated samples by western blot analysis.A. 30 ?g HepG2 whole cell extractB. 30 ?g HepG2 whole cell extract (1% O2 treatment for 24 hr)7.5 % SDS-PAGEHIF1 alpha antibody (GTX127309) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis.A. 30 ?g HepG2 whole cell lysate/extract (untreated) B.30 ?g HepG2 whole cell lysate/extract ( 200 £gM CoCl2 treatment for 24 hr)C. 30 ?g HepG2 whole cell lysate/extract (500 £gM CoCl2 treatment for 24 hr)7.5% SDS-PAGEHIF1 alpha antibody (GTX127309) dilution: 1:5000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) at a dilution of 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) NIH-3T3 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) MCF-7 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) MCF-7 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HIF1 alpha antibody detects HIF1 alpha protein at nucleus in CoCl2-treated samples by confocal immunofluorescent analysis.Samples: Untreated (left panel) and 500£gM CoCl2 treated (right panel) HeLa cells for 24 hrs were fixed in 4% paraformaldehyde for 15 min Green: HIF1alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:1000 Red: Alpha-tubulin, a cytoskeleton marker, stained by Alpha-tubulin antibody (GTX11304) diluted at 1:2500Blue: Hoechst 33342 staining.Scale bar = 10 £gm.Images captured by Olympus FV10i Confocal Laser Scanning Microscope
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIF1 alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:200.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIF1 alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Scale bar = 10 £gm.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIF1 alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HIF1 alpha antibody immunoprecipitates HIF1 alpha protein in IP experiments. IP Sample: 950 µg whole cell lysate/extract of HIF1 alpha protein expressing HeLa cells. A. 20 ?g whole cell lysate/extract of transfected HeLa cells B. Control with 2.5 ?g of preimmune rabbit IgG C. Immunoprecipitation of HIF1 alpha protein by 2.5 ?g of HIF1 alpha antibody (GTX127309) 7.5% SDS-PAGE The immunoprecipitated HIF1 alpha protein was detected by HIF1 alpha antibody (GTX127309) diluted at 1:10000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
- Validation comment
- IP
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of HIF1 alpha protein from HepG2 whole cell extracts treated with 500 £gM CoCl2 for 24 hr using 5 £gg of HIF1 alpha antibody (GTX127309).Western blot analysis was performed using HIF1 alpha antibody (GTX127309).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HIF1 alpha antibody detects HIF1 alpha protein at nucleus on human kidney cancer by immunohistochemical analysis. Sample: Paraffin-embedded human kidney cancer. HIF1 alpha antibody (GTX127309) dilution: 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HIF-1 alpha antibody immunoprecipitates HIF-1 alpha protein-DNA complex in ChIP experiments. ChIP Sample: HepG2 whole cell lysate/extract under hypoxia (1%O2) treatment 24hr A. 5 ?g preimmune rabbit IgGB. 5 ?g of HIF-1 alpha antibody (GTX127309)The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.
- Validation comment
- ChIP
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ChIP was performed with HepG2 chromatin extract and 5 £gg of either normal rabbit IgG or anti-HIF1 alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.
