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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-296 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-296, RRID:AB_10002075
- Product name
- Mouse Monoclonal HIF-1 alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references hERG1 and HIF-2α Behave as Biomarkers of Positive Response to Bevacizumab in Metastatic Colorectal Cancer Patients.
Detection of Hypoxia and HIF in Paraffin-Embedded Tumor Tissues.
Hif1α Deletion Limits Tissue Regeneration via Aberrant B Cell Accumulation in Experimental Pancreatitis.
Hif1a Deletion Reveals Pro-Neoplastic Function of B Cells in Pancreatic Neoplasia.
Proteasomal activity in placentas from women with preeclampsia and intrauterine growth restriction: implications for expression of HIF-alpha proteins.
(2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide (BiPS), a matrix metalloprotease inhibitor, is a novel and potent activator of hypoxia-inducible factors.
Silencing of hypoxia inducible factor-1alpha by RNA interference attenuates human glioma cell growth in vivo.
Cell-type-specific regulation of degradation of hypoxia-inducible factor 1 alpha: role of subcellular compartmentalization.
Inhibition of hypoxia inducible factor-1alpha (HIF-1alpha) decreases vascular endothelial growth factor (VEGF) secretion and tumor growth in malignant gliomas.
Iorio J, Lastraioli E, Tofani L, Petroni G, Antonuzzo L, Messerini L, Perrone G, Caputo D, Francesconi M, Amato MM, Cadei M, Arcangeli G, Villanacci V, Boni L, Coppola R, Di Costanzo F, Arcangeli A
Translational oncology 2020 Mar;13(3):100740
Translational oncology 2020 Mar;13(3):100740
Detection of Hypoxia and HIF in Paraffin-Embedded Tumor Tissues.
Li F, Lee KE, Simon MC
Methods in molecular biology (Clifton, N.J.) 2018;1742:277-282
Methods in molecular biology (Clifton, N.J.) 2018;1742:277-282
Hif1α Deletion Limits Tissue Regeneration via Aberrant B Cell Accumulation in Experimental Pancreatitis.
Lee KE, Spata M, Maduka R, Vonderheide RH, Simon MC
Cell reports 2018 Jun 19;23(12):3457-3464
Cell reports 2018 Jun 19;23(12):3457-3464
Hif1a Deletion Reveals Pro-Neoplastic Function of B Cells in Pancreatic Neoplasia.
Lee KE, Spata M, Bayne LJ, Buza EL, Durham AC, Allman D, Vonderheide RH, Simon MC
Cancer discovery 2016 Mar;6(3):256-69
Cancer discovery 2016 Mar;6(3):256-69
Proteasomal activity in placentas from women with preeclampsia and intrauterine growth restriction: implications for expression of HIF-alpha proteins.
Rajakumar A, Michael HM, Daftary A, Jeyabalan A, Gilmour C, Conrad KP
Placenta 2008 Mar;29(3):290-9
Placenta 2008 Mar;29(3):290-9
(2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide (BiPS), a matrix metalloprotease inhibitor, is a novel and potent activator of hypoxia-inducible factors.
Lauzier MC, Robitaille GA, Chan DA, Giaccia AJ, Richard DE
Molecular pharmacology 2008 Jul;74(1):282-8
Molecular pharmacology 2008 Jul;74(1):282-8
Silencing of hypoxia inducible factor-1alpha by RNA interference attenuates human glioma cell growth in vivo.
Gillespie DL, Whang K, Ragel BT, Flynn JR, Kelly DA, Jensen RL
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Apr 15;13(8):2441-8
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Apr 15;13(8):2441-8
Cell-type-specific regulation of degradation of hypoxia-inducible factor 1 alpha: role of subcellular compartmentalization.
Zheng X, Ruas JL, Cao R, Salomons FA, Cao Y, Poellinger L, Pereira T
Molecular and cellular biology 2006 Jun;26(12):4628-41
Molecular and cellular biology 2006 Jun;26(12):4628-41
Inhibition of hypoxia inducible factor-1alpha (HIF-1alpha) decreases vascular endothelial growth factor (VEGF) secretion and tumor growth in malignant gliomas.
Jensen RL, Ragel BT, Whang K, Gillespie D
Journal of neuro-oncology 2006 Jul;78(3):233-47
Journal of neuro-oncology 2006 Jul;78(3):233-47
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HIF-1 alpha Antibody (HA111) [NB100-296] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.
