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LearnALX-804-216-R100
antibody from Enzo Life Sciences
Targeting: HIF1A
bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8
Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [2]
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- Product number
- ALX-804-216-R100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-216-R100, RRID:AB_2051592
- Product name
- HIF-1_ monoclonal antibody (mgc3)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Bovine, Porcine, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- mgc3
- Vial size
- 100 μl
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Preconditional activation of hypoxia-inducible factors ameliorates ischemic acute renal failure.
General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1alpha.
Induction and nuclear translocation of hypoxia-inducible factor-1 (HIF-1): heterodimerization with ARNT is not necessary for nuclear accumulation of HIF-1alpha.
Bernhardt WM, Câmpean V, Kany S, Jürgensen JS, Weidemann A, Warnecke C, Arend M, Klaus S, Günzler V, Amann K, Willam C, Wiesener MS, Eckardt KU
Journal of the American Society of Nephrology : JASN 2006 Jul;17(7):1970-8
Journal of the American Society of Nephrology : JASN 2006 Jul;17(7):1970-8
General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1alpha.
Camenisch G, Tini M, Chilov D, Kvietikova I, Srinivas V, Caro J, Spielmann P, Wenger RH, Gassmann M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1999 Jan;13(1):81-8
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1999 Jan;13(1):81-8
Induction and nuclear translocation of hypoxia-inducible factor-1 (HIF-1): heterodimerization with ARNT is not necessary for nuclear accumulation of HIF-1alpha.
Chilov D, Camenisch G, Kvietikova I, Ziegler U, Gassmann M, Wenger RH
Journal of cell science 1999 Apr;112 ( Pt 8):1203-12
Journal of cell science 1999 Apr;112 ( Pt 8):1203-12
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Figure A: Western blot using MAb to Hypoxia-inducible Factor 1_ (mgc3) (Prod. No. ALX-804-216). Method: Nuclear extracts (25µg) from normoxic (20% O2) and hypoxic (1% O2) human HeLa and mouse Hepa1 cells were subjected to SDS-PAGE and HIF-1_ was detected in Western blots using MAb mgc3 (1:1'000). Note that mgc3 detects denatured HIF-1_ only in human cells in Western blot analysis [1].
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Figure B: Electrophoretic mobility shift assay (EMSA) using MAb to Hypoxia-inducible Factor 1_ (mgc3) (Prod. No. ALX-804-216). Method: Nuclear extracts (5µg) from normoxic and hypoxic human HeLa and mouse wild-type (Hepa1) and ARNT-mutant (Hepa1C4) hepatoma cells were analyzed by EMSA. MAb mgc3 supershifted the HIF-1 heterodimer in HeLa and Hepa1 cells. No HIF DNA-binding activity was detected in ARNT-mutant Hepa1C4 cells. In contrast to Western blot analysis, mgc3 was able to detect also non-human HIF-1_ in EMSA [1,2].
