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LearnAMM80975
antibody from EnkiLife Biotech Co., Ltd.
Targeting: HIF1A
bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AMM80975 - Provider product page
- Provider
- EnkiLife Biotech Co., Ltd.
- Product name
- HIF1A Mouse Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Affinity Purification
- Reactivity
- Human, Mouse, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- Monoclonal
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western blot analysis using HIF1A mouse mAb against Cos7 (1), Hela (2), Jurkat (3), RAJI (4) and NIH/3T3 (5) cell lysate.
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Hela cells using HIF1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human liver cancer tissues (left) and lung cancer tissues (right) using HIF1A mouse mAb with DAB staining.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues (left) and brain tumor tissues (right) using HIF1A mouse mAb with DAB staining.
