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LearnAP09087SU-N
antibody from Acris Antibodies GmbH
Targeting: HIF1A
bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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- Product number
- AP09087SU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09087SU-N, RRID:AB_2035627
- Product name
- anti HIF1A / HIF1 alpha (Hydroxy P564)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to a region surrounding the P564 of human HIF-1 alpha
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 85 mg/ml (by Refractometry)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using anti-HIF-1alpha HYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF1-alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF1-alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF1-alpha. The asterisk marks a band approximately 110 kDa in size corresponding to HIF1-alpha. The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO.
