Antibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- AF1935 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse and rat HIF-1 alpha/HIF1A.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q16665.1
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Co-Expression of VEGF and IL-6 Family Cytokines is Associated with Decreased Survival in HER2 Negative Breast Cancer Patients: Subtype-Specific IL-6 Family Cytokine-Mediated VEGF Secretion.
PRKAA1/AMPKα1-driven glycolysis in endothelial cells exposed to disturbed flow protects against atherosclerosis.
HIF prolyl hydroxylase inhibition protects skeletal muscle from eccentric contraction-induced injury.
Endothelial adenosine A2a receptor-mediated glycolysis is essential for pathological retinal angiogenesis.
Motor neurons control blood vessel patterning in the developing spinal cord.
Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells.
Forkhead box O3 (FoxO3) regulates kidney tubular autophagy following urinary tract obstruction.
Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo(-/-) mice.
Identification of β-Dystrobrevin as a Direct Target of miR-143: Involvement in Early Stages of Neural Differentiation.
Heregulin/ErbB3 Signaling Enhances CXCR4-Driven Rac1 Activation and Breast Cancer Cell Motility via Hypoxia-Inducible Factor 1α.
Stress-resistant Translation of Cathepsin L mRNA in Breast Cancer Progression.
High Glucose Up-regulates ADAM17 through HIF-1α in Mesangial Cells.
Warburg-like Glycolysis and Lactate Shuttle in Mouse Decidua during Early Pregnancy.
The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells.
Myeloid cell leukemia-1 (Mc1-1) is a candidate target gene of hypoxia-inducible factor-1 (HIF-1) in the testis.
HIF-1-mediated up-regulation of cardiotrophin-1 is involved in the survival response of cardiomyocytes to hypoxia.
Development of an in vitro model of myotube ischemia.
HER-2 signaling, acquisition of growth factor independence, and regulation of biological networks associated with cell transformation.
Hypoxia induces leptin gene expression and secretion in human preadipocytes: differential effects of hypoxia on adipokine expression by preadipocytes.
The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha (HIF-1alpha) activity: involvement in myeloma-induced angiogenesis.
Tawara K, Scott H, Emathinger J, Ide A, Fox R, Greiner D, LaJoie D, Hedeen D, Nandakumar M, Oler AJ, Holzer R, Jorcyk C
Translational oncology 2019 Feb;12(2):245-255
Translational oncology 2019 Feb;12(2):245-255
PRKAA1/AMPKα1-driven glycolysis in endothelial cells exposed to disturbed flow protects against atherosclerosis.
Yang Q, Xu J, Ma Q, Liu Z, Sudhahar V, Cao Y, Wang L, Zeng X, Zhou Y, Zhang M, Xu Y, Wang Y, Weintraub NL, Zhang C, Fukai T, Wu C, Huang L, Han Z, Wang T, Fulton DJ, Hong M, Huo Y
Nature communications 2018 Nov 7;9(1):4667
Nature communications 2018 Nov 7;9(1):4667
HIF prolyl hydroxylase inhibition protects skeletal muscle from eccentric contraction-induced injury.
Billin AN, Honeycutt SE, McDougal AV, Kerr JP, Chen Z, Freudenberg JM, Rajpal DK, Luo G, Kramer HF, Geske RS, Fang F, Yao B, Clark RV, Lepore J, Cobitz A, Miller R, Nosaka K, Hinken AC, Russell AJ
Skeletal muscle 2018 Nov 13;8(1):35
Skeletal muscle 2018 Nov 13;8(1):35
Endothelial adenosine A2a receptor-mediated glycolysis is essential for pathological retinal angiogenesis.
Liu Z, Yan S, Wang J, Xu Y, Wang Y, Zhang S, Xu X, Yang Q, Zeng X, Zhou Y, Gu X, Lu S, Fu Z, Fulton DJ, Weintraub NL, Caldwell RB, Zhang W, Wu C, Liu XL, Chen JF, Ahmad A, Kaddour-Djebbar I, Al-Shabrawey M, Li Q, Jiang X, Sun Y, Sodhi A, Smith L, Hong M, Huo Y
Nature communications 2017 Sep 19;8(1):584
Nature communications 2017 Sep 19;8(1):584
Motor neurons control blood vessel patterning in the developing spinal cord.
Himmels P, Paredes I, Adler H, Karakatsani A, Luck R, Marti HH, Ermakova O, Rempel E, Stoeckli ET, Ruiz de Almodóvar C
Nature communications 2017 Mar 6;8:14583
Nature communications 2017 Mar 6;8:14583
Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells.
Li X, Lu J, Kan Q, Li X, Fan Q, Li Y, Huang R, Slipicevic A, Dong HP, Eide L, Wang J, Zhang H, Berge V, Goscinski MA, Kvalheim G, Nesland JM, Suo Z
Scientific reports 2017 Jul 11;7(1):5081
Scientific reports 2017 Jul 11;7(1):5081
Forkhead box O3 (FoxO3) regulates kidney tubular autophagy following urinary tract obstruction.
Li L, Zviti R, Ha C, Wang ZV, Hill JA, Lin F
The Journal of biological chemistry 2017 Aug 18;292(33):13774-13783
The Journal of biological chemistry 2017 Aug 18;292(33):13774-13783
Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo(-/-) mice.
Campbell EJ, Vissers MC, Dachs GU
Hypoxia (Auckland, N.Z.) 2016;4:41-52
Hypoxia (Auckland, N.Z.) 2016;4:41-52
Identification of β-Dystrobrevin as a Direct Target of miR-143: Involvement in Early Stages of Neural Differentiation.
Quaranta MT, Spinello I, Paolillo R, Macchia G, Boe A, Ceccarini M, Labbaye C, Macioce P
PloS one 2016;11(5):e0156325
PloS one 2016;11(5):e0156325
Heregulin/ErbB3 Signaling Enhances CXCR4-Driven Rac1 Activation and Breast Cancer Cell Motility via Hypoxia-Inducible Factor 1α.
Lopez-Haber C, Barrio-Real L, Casado-Medrano V, Kazanietz MG
Molecular and cellular biology 2016 Aug 1;36(15):2011-26
Molecular and cellular biology 2016 Aug 1;36(15):2011-26
Stress-resistant Translation of Cathepsin L mRNA in Breast Cancer Progression.
Tholen M, Wolanski J, Stolze B, Chiabudini M, Gajda M, Bronsert P, Stickeler E, Rospert S, Reinheckel T
The Journal of biological chemistry 2015 Jun 19;290(25):15758-15769
The Journal of biological chemistry 2015 Jun 19;290(25):15758-15769
High Glucose Up-regulates ADAM17 through HIF-1α in Mesangial Cells.
Li R, Uttarwar L, Gao B, Charbonneau M, Shi Y, Chan JS, Dubois CM, Krepinsky JC
The Journal of biological chemistry 2015 Aug 28;290(35):21603-14
The Journal of biological chemistry 2015 Aug 28;290(35):21603-14
Warburg-like Glycolysis and Lactate Shuttle in Mouse Decidua during Early Pregnancy.
Zuo RJ, Gu XW, Qi QR, Wang TS, Zhao XY, Liu JL, Yang ZM
The Journal of biological chemistry 2015 Aug 28;290(35):21280-91
The Journal of biological chemistry 2015 Aug 28;290(35):21280-91
The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells.
Liang D, Ma Y, Liu J, Trope CG, Holm R, Nesland JM, Suo Z
BMC cancer 2012 May 29;12:201
BMC cancer 2012 May 29;12:201
Myeloid cell leukemia-1 (Mc1-1) is a candidate target gene of hypoxia-inducible factor-1 (HIF-1) in the testis.
Palladino MA, Shah A, Tyson R, Horvath J, Dugan C, Karpodinis M
Reproductive biology and endocrinology : RB&E 2012 Dec 5;10:104
Reproductive biology and endocrinology : RB&E 2012 Dec 5;10:104
HIF-1-mediated up-regulation of cardiotrophin-1 is involved in the survival response of cardiomyocytes to hypoxia.
Robador PA, San José G, Rodríguez C, Guadall A, Moreno MU, Beaumont J, Fortuño A, Díez J, Martínez-González J, Zalba G
Cardiovascular research 2011 Nov 1;92(2):247-55
Cardiovascular research 2011 Nov 1;92(2):247-55
Development of an in vitro model of myotube ischemia.
Joshi D, Patel H, Baker DM, Shiwen X, Abraham DJ, Tsui JC
Laboratory investigation; a journal of technical methods and pathology 2011 Aug;91(8):1241-52
Laboratory investigation; a journal of technical methods and pathology 2011 Aug;91(8):1241-52
HER-2 signaling, acquisition of growth factor independence, and regulation of biological networks associated with cell transformation.
Bollig-Fischer A, Dziubinski M, Boyer A, Haddad R, Giroux CN, Ethier SP
Cancer research 2010 Oct 15;70(20):7862-73
Cancer research 2010 Oct 15;70(20):7862-73
Hypoxia induces leptin gene expression and secretion in human preadipocytes: differential effects of hypoxia on adipokine expression by preadipocytes.
Wang B, Wood IS, Trayhurn P
The Journal of endocrinology 2008 Jul;198(1):127-34
The Journal of endocrinology 2008 Jul;198(1):127-34
The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha (HIF-1alpha) activity: involvement in myeloma-induced angiogenesis.
Colla S, Tagliaferri S, Morandi F, Lunghi P, Donofrio G, Martorana D, Mancini C, Lazzaretti M, Mazzera L, Ravanetti L, Bonomini S, Ferrari L, Miranda C, Ladetto M, Neri TM, Neri A, Greco A, Mangoni M, Bonati A, Rizzoli V, Giuliani N
Blood 2007 Dec 15;110(13):4464-75
Blood 2007 Dec 15;110(13):4464-75
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human HIF-1 alpha/HIF1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 μM CoCl2 for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Antigen-affinity Purified Polyclonal Antibody, followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF-1 alpha/HIF1A at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human HIF-1 alpha/HIF1A by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O2), loaded at 0.2 mg/mL. A specific band was detected for HIF-1 alpha/HIF1A at approximately 115 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HIF-1 alpha/HIF1A Specificity is Shown by Immunocytochemistry in Knockout Cell Line. HIF-1 alpha/HIF1A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO but is not detected in HIF-1 alpha/HIF1A knockout (KO) HeLa Human Cell Line cell line using Goat Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of HIF-1 alpha/HIF1A-regulated Genes by Chromatin Immunoprecipitation. Mouse primary kidney cells treated with 150 μM CoCl2 for overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HIF-1 alpha/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The epo promoter was detected by standard PCR.