IC1935P

antibody from R&D Systems

Targeting: HIF1A bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC1935P

Provider

R&D Systems

Product name

Human/Mouse HIF-1 alpha/HIF1A PE-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Recognizes human and mouse HIF-1 alpha in direct ELISAs.

Reactivity

Human, Mouse

Host

Mouse

Antigen sequence

Q16665.1


Isotype

IgG

Antibody clone number

241812

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

Induction of HIF-1α by HIV-1 Infection in CD4⁺ T Cells Promotes Viral Replication and Drives Extracellular Vesicle-Mediated Inflammation.

Duette G, Pereyra Gerber P, Rubione J, Perez PS, Landay AL, Crowe SM, Liao Z, Witwer KW, Holgado MP, Salido J, Geffner J, Sued O, Palmer CS, Ostrowski M
mBio 2018 Sep 11;9(5)

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Glucocorticoid receptor promotes the function of myeloid-derived suppressor cells by suppressing HIF1α-dependent glycolysis.

Lu Y, Liu H, Bi Y, Yang H, Li Y, Wang J, Zhang Z, Wang Y, Li C, Jia A, Han L, Hu Y, Zhao Y, Wang R, Liu G
Cellular & molecular immunology 2018 Jun;15(6):618-629

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of HIF-1 alpha in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line treated with CoCl₂ was stained with Mouse Anti-Human/Mouse HIF-1 alpha PE-conjugated Monoclonal Antibody (Catalog # IC1935P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.