Antibody data
- Antibody Data
- Antigen structure
- References [25]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB1536 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat HIF-1 alpha/HIF1A.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q16665.1
- Isotype
- IgG
- Antibody clone number
- 241809
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip.
Protective effect of zinc preconditioning against renal ischemia reperfusion injury is dose dependent.
Metabolic changes and inflammation in cultured astrocytes from the 5xFAD mouse model of Alzheimer's disease: Alleviation by pantethine.
XIAP upregulates expression of HIF target genes by targeting HIF1α for Lys63-linked polyubiquitination.
Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency.
VHL promotes immune response against renal cell carcinoma via NF-κB-dependent regulation of VCAM-1.
Ferrochelatase is a therapeutic target for ocular neovascularization.
Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia.
Silencing HIF-1α induces TET2 expression and augments ascorbic acid induced 5-hydroxymethylation of DNA in human metastatic melanoma cells.
HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia.
Chronic intermittent hypoxia induces cardiac inflammation and dysfunction in a rat obstructive sleep apnea model.
NQO1 inhibits proteasome-mediated degradation of HIF-1α.
Phosphoribosylation of Ubiquitin Promotes Serine Ubiquitination and Impairs Conventional Ubiquitination.
Leptin directly promotes T-cell glycolytic metabolism to drive effector T-cell differentiation in a mouse model of autoimmunity.
Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.
Panobinostat reduces hypoxia-induced cisplatin resistance of non-small cell lung carcinoma cells via HIF-1α destabilization.
NF-κB enhances hypoxia-driven T-cell immunosuppression via upregulation of adenosine A(2A) receptors.
HIF-1-PHD2 axis controls expression of syndecan 4 in nucleus pulposus cells.
The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability.
Evolutionary conserved regulation of HIF-1β by NF-κB.
Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1α in MSCs.
The von Hippel-Lindau Chuvash mutation promotes pulmonary hypertension and fibrosis in mice.
CYR61 and alphaVbeta5 integrin cooperate to promote invasion and metastasis of tumors growing in preirradiated stroma.
Oxygen tension regulates preosteocyte maturation and mineralization.
Nucleus pulposus cells express HIF-1 alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment.
Jalili-Firoozinezhad S, Prantil-Baun R, Jiang A, Potla R, Mammoto T, Weaver JC, Ferrante TC, Kim HJ, Cabral JMS, Levy O, Ingber DE
Cell death & disease 2018 Feb 14;9(2):223
Cell death & disease 2018 Feb 14;9(2):223
Protective effect of zinc preconditioning against renal ischemia reperfusion injury is dose dependent.
Rao K, Sethi K, Ischia J, Gibson L, Galea L, Xiao L, Yim M, Chang M, Papa N, Bolton D, Shulkes A, Baldwin GS, Patel O
PloS one 2017;12(7):e0180028
PloS one 2017;12(7):e0180028
Metabolic changes and inflammation in cultured astrocytes from the 5xFAD mouse model of Alzheimer's disease: Alleviation by pantethine.
van Gijsel-Bonnello M, Baranger K, Benech P, Rivera S, Khrestchatisky M, de Reggi M, Gharib B
PloS one 2017;12(4):e0175369
PloS one 2017;12(4):e0175369
XIAP upregulates expression of HIF target genes by targeting HIF1α for Lys63-linked polyubiquitination.
Park CV, Ivanova IG, Kenneth NS
Nucleic acids research 2017 Sep 19;45(16):9336-9347
Nucleic acids research 2017 Sep 19;45(16):9336-9347
Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency.
Sone M, Morone N, Nakamura T, Tanaka A, Okita K, Woltjen K, Nakagawa M, Heuser JE, Yamada Y, Yamanaka S, Yamamoto T
Cell metabolism 2017 May 2;25(5):1103-1117.e6
Cell metabolism 2017 May 2;25(5):1103-1117.e6
VHL promotes immune response against renal cell carcinoma via NF-κB-dependent regulation of VCAM-1.
Labrousse-Arias D, Martínez-Alonso E, Corral-Escariz M, Bienes-Martínez R, Berridy J, Serrano-Oviedo L, Conde E, García-Bermejo ML, Giménez-Bachs JM, Salinas-Sánchez AS, Sánchez-Prieto R, Yao M, Lasa M, Calzada MJ
The Journal of cell biology 2017 Mar 6;216(3):835-847
The Journal of cell biology 2017 Mar 6;216(3):835-847
Ferrochelatase is a therapeutic target for ocular neovascularization.
Basavarajappa HD, Sulaiman RS, Qi X, Shetty T, Sheik Pran Babu S, Sishtla KL, Lee B, Quigley J, Alkhairy S, Briggs CM, Gupta K, Tang B, Shadmand M, Grant MB, Boulton ME, Seo SY, Corson TW
EMBO molecular medicine 2017 Jun;9(6):786-801
EMBO molecular medicine 2017 Jun;9(6):786-801
Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia.
Hernansanz-Agustín P, Ramos E, Navarro E, Parada E, Sánchez-López N, Peláez-Aguado L, Cabrera-García JD, Tello D, Buendia I, Marina A, Egea J, López MG, Bogdanova A, Martínez-Ruiz A
Redox biology 2017 Aug;12:1040-1051
Redox biology 2017 Aug;12:1040-1051
Silencing HIF-1α induces TET2 expression and augments ascorbic acid induced 5-hydroxymethylation of DNA in human metastatic melanoma cells.
Fischer AP, Miles SL
Biochemical and biophysical research communications 2017 Aug 19;490(2):176-181
Biochemical and biophysical research communications 2017 Aug 19;490(2):176-181
HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia.
Wang P, Lan C, Xiong S, Zhao X, Shan Y, Hu R, Wan W, Yu S, Liao B, Li G, Wang J, Zou D, Chen B, Feng H, Wu N
Oncotarget 2017 Apr 25;8(17):28074-28092
Oncotarget 2017 Apr 25;8(17):28074-28092
Chronic intermittent hypoxia induces cardiac inflammation and dysfunction in a rat obstructive sleep apnea model.
Wei Q, Bian Y, Yu F, Zhang Q, Zhang G, Li Y, Song S, Ren X, Tong J
Journal of biomedical research 2016 Nov;30(6):490-495
Journal of biomedical research 2016 Nov;30(6):490-495
NQO1 inhibits proteasome-mediated degradation of HIF-1α.
Oh ET, Kim JW, Kim JM, Kim SJ, Lee JS, Hong SS, Goodwin J, Ruthenborg RJ, Jung MG, Lee HJ, Lee CH, Park ES, Kim C, Park HJ
Nature communications 2016 Dec 14;7:13593
Nature communications 2016 Dec 14;7:13593
Phosphoribosylation of Ubiquitin Promotes Serine Ubiquitination and Impairs Conventional Ubiquitination.
Bhogaraju S, Kalayil S, Liu Y, Bonn F, Colby T, Matic I, Dikic I
Cell 2016 Dec 1;167(6):1636-1649.e13
Cell 2016 Dec 1;167(6):1636-1649.e13
Leptin directly promotes T-cell glycolytic metabolism to drive effector T-cell differentiation in a mouse model of autoimmunity.
Gerriets VA, Danzaki K, Kishton RJ, Eisner W, Nichols AG, Saucillo DC, Shinohara ML, MacIver NJ
European journal of immunology 2016 Aug;46(8):1970-83
European journal of immunology 2016 Aug;46(8):1970-83
Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.
Sun Y, George J, Rocha S
PloS one 2015;10(4):e0123649
PloS one 2015;10(4):e0123649
Panobinostat reduces hypoxia-induced cisplatin resistance of non-small cell lung carcinoma cells via HIF-1α destabilization.
Fischer C, Leithner K, Wohlkoenig C, Quehenberger F, Bertsch A, Olschewski A, Olschewski H, Hrzenjak A
Molecular cancer 2015 Jan 21;14:4
Molecular cancer 2015 Jan 21;14:4
NF-κB enhances hypoxia-driven T-cell immunosuppression via upregulation of adenosine A(2A) receptors.
Bruzzese L, Fromonot J, By Y, Durand-Gorde JM, Condo J, Kipson N, Guieu R, Fenouillet E, Ruf J
Cellular signalling 2014 May;26(5):1060-7
Cellular signalling 2014 May;26(5):1060-7
HIF-1-PHD2 axis controls expression of syndecan 4 in nucleus pulposus cells.
Fujita N, Hirose Y, Tran CM, Chiba K, Miyamoto T, Toyama Y, Shapiro IM, Risbud MV
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Jun;28(6):2455-65
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Jun;28(6):2455-65
The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability.
Bett JS, Ibrahim AF, Garg AK, Kelly V, Pedrioli P, Rocha S, Hay RT
The Biochemical journal 2013 Apr 15;451(2):185-94
The Biochemical journal 2013 Apr 15;451(2):185-94
Evolutionary conserved regulation of HIF-1β by NF-κB.
van Uden P, Kenneth NS, Webster R, Müller HA, Mudie S, Rocha S
PLoS genetics 2011 Jan 27;7(1):e1001285
PLoS genetics 2011 Jan 27;7(1):e1001285
Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1α in MSCs.
Liu H, Xue W, Ge G, Luo X, Li Y, Xiang H, Ding X, Tian P, Tian X
Biochemical and biophysical research communications 2010 Oct 29;401(4):509-15
Biochemical and biophysical research communications 2010 Oct 29;401(4):509-15
The von Hippel-Lindau Chuvash mutation promotes pulmonary hypertension and fibrosis in mice.
Hickey MM, Richardson T, Wang T, Mosqueira M, Arguiri E, Yu H, Yu QC, Solomides CC, Morrisey EE, Khurana TS, Christofidou-Solomidou M, Simon MC
The Journal of clinical investigation 2010 Mar;120(3):827-39
The Journal of clinical investigation 2010 Mar;120(3):827-39
CYR61 and alphaVbeta5 integrin cooperate to promote invasion and metastasis of tumors growing in preirradiated stroma.
Monnier Y, Farmer P, Bieler G, Imaizumi N, Sengstag T, Alghisi GC, Stehle JC, Ciarloni L, Andrejevic-Blant S, Moeckli R, Mirimanoff RO, Goodman SL, Delorenzi M, Rüegg C
Cancer research 2008 Sep 15;68(18):7323-31
Cancer research 2008 Sep 15;68(18):7323-31
Oxygen tension regulates preosteocyte maturation and mineralization.
Zahm AM, Bucaro MA, Srinivas V, Shapiro IM, Adams CS
Bone 2008 Jul;43(1):25-31
Bone 2008 Jul;43(1):25-31
Nucleus pulposus cells express HIF-1 alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment.
Risbud MV, Guttapalli A, Stokes DG, Hawkins D, Danielson KG, Schaer TP, Albert TJ, Shapiro IM
Journal of cellular biochemistry 2006 May 1;98(1):152-9
Journal of cellular biochemistry 2006 May 1;98(1):152-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat HIF-1 alpha by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Balb-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 μM CoCl2 for 8 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HIF-1 alpha Monoclonal Antibody, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human HIF-1 alpha by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O2), loaded at 0.2 mg/mL. A specific band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat HIF-1 alpha Monoclonal Antibody (Catalog # MAB1536). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human HIF-1 alpha Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO overnight. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HIF-1 alpha Monoclonal Antibody (Catalog # MAB1536) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HIF-1 alpha Specificity is Shown by Immunocytochemistry in Knockout Cell Line. HIF-1 alpha was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO but is not detected in HIF-1 alpha knockout (KO) HeLa Human Cell Line cell line using Mouse Anti-Human/Mouse/Rat HIF-1 alpha Monoclonal Antibody (Catalog # MAB1536) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HIF-1 alpha in Human Kidney. HIF-1 alpha was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat HIF-1 alpha Monoclonal Antibody (Catalog # MAB1536) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.