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Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunohistochemistry [1]
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- Product number
- MAB1935 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse HIF-1 alpha/HIF1A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Recognizes human and mouse HIF-1 alpha/HIF1A in direct ELISAs.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q16665.1- Isotype
- IgG
- Antibody clone number
- 241812
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Celastrol mediates Th17 and Treg cell generation via metabolic signaling.
Dendritic cell SIRT1-HIF1α axis programs the differentiation of CD4+ T cells through IL-12 and TGF-β1.
Hypoxia promotes the proliferation of cervical carcinoma cells through stimulating the secretion of IL-8.
Loss of VHL in mesenchymal progenitors of the limb bud alters multiple steps of endochondral bone development.
Effects of sustained antiangiogenic therapy in multistage prostate cancer in TRAMP model.
Zhang J, Shan J, Chen X, Li S, Long D, Li Y
Biochemical and biophysical research communications 2018 Mar 11;497(3):883-889
Biochemical and biophysical research communications 2018 Mar 11;497(3):883-889
Dendritic cell SIRT1-HIF1α axis programs the differentiation of CD4+ T cells through IL-12 and TGF-β1.
Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 3;112(9):E957-65
Proceedings of the National Academy of Sciences of the United States of America 2015 Mar 3;112(9):E957-65
Hypoxia promotes the proliferation of cervical carcinoma cells through stimulating the secretion of IL-8.
Liu LB, Xie F, Chang KK, Li MQ, Meng YH, Wang XH, Li H, Li DJ, Yu JJ
International journal of clinical and experimental pathology 2014;7(2):575-83
International journal of clinical and experimental pathology 2014;7(2):575-83
Loss of VHL in mesenchymal progenitors of the limb bud alters multiple steps of endochondral bone development.
Mangiavini L, Merceron C, Araldi E, Khatri R, Gerard-O'Riley R, Wilson TL, Rankin EB, Giaccia AJ, Schipani E
Developmental biology 2014 Sep 1;393(1):124-36
Developmental biology 2014 Sep 1;393(1):124-36
Effects of sustained antiangiogenic therapy in multistage prostate cancer in TRAMP model.
Isayeva T, Chanda D, Kallman L, Eltoum IE, Ponnazhagan S
Cancer research 2007 Jun 15;67(12):5789-97
Cancer research 2007 Jun 15;67(12):5789-97
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HIF-1 alpha in Human Kidney Cancer Tissue. HIF-1 alpha was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Mouse Anti-Human/Mouse HIF-1 alpha Monoclonal Antibody (Catalog # MAB1935) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
