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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- 41-3500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOTCH1 Monoclonal Antibody (mN1A)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody is specific for the Notch1 (neurogenic locus notch homolog protein1, Motch A, mT14, p300) protein. This antibody gives a band of ~65 kDa on Western blot analysis using Notch1-IC OP transfected cells. Western blot analysis using Jurkat cells expresses the target band at ~110-120 kDa, with an additional band occasionally observed at ~220kDa. This antibody does not cross-react with Notch 2,3, and 4 as assessed by Western blot analysis.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- mN1A
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references LncRNA DIGIT Accelerates Tube Formation of Vascular Endothelial Cells by Sponging miR-134.
Characterization of two distinct lymphoproliferative diseases caused by ectopic expression of the Notch ligand DLL4 on T cells.
Miao C, Cao H, Zhang Y, Guo X, Wang Z, Wang J
International heart journal 2018 Sep 26;59(5):1086-1095
International heart journal 2018 Sep 26;59(5):1086-1095
Characterization of two distinct lymphoproliferative diseases caused by ectopic expression of the Notch ligand DLL4 on T cells.
Xiong H, Maraver A, Latkowski JA, Henderson T, Schlessinger K, Ding Y, Shen J, Tadokoro CE, Lafaille JJ
PloS one 2013;8(12):e84841
PloS one 2013;8(12):e84841
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Knockout of NOTCH1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR682588_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of NOTCH1 was performed by loading 50 µg of MDA-MB-231 wild type (Lane 1) and MDA-MB-231 NOTCH1 KO (Lane 2) whole cell extracts. The samples were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-NOTCH1 Monoclonal Antibody (mN1A) (Product # 41-3500, 1:500 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to NOTCH1.
