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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA300836 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA300836, RRID:AB_2153343
- Product name
- Rabbit Monoclonal Antibody against NOTCH1 (Clone EP1238Y)
- Antibody type
- Monoclonal
- Description
- Rabbit Monoclonal Antibody against NOTCH1 (Clone EP1238Y)
- Reactivity
- Bovine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- NOTCH1
- Isotype
- IgG
- Antibody clone number
- EP1238Y
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot - Notch1 antibody [EP1238Y]; Anti-Notch1 [EP1238Y] antibody at 1/500 dilution + Hek293 cell lysate at 10 ug.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Observed band size : 125 kDa .
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ICC/IF image of TA300836 stained HepG2 cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin-embedded sections) - Notch1 antibody [EP1238Y]; TA300836, at a 1/50 dilution, staining human Notch1 in fetal lung tissue, using Immunohistochemistry, Paraffin embedded tissue.
- Validation comment
- IHC
