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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- TA336494 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal Notch-1 Antibody (mN1A)
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal Notch-1 Antibody (mN1A)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NOTCH1
- Isotype
- IgG
- Antibody clone number
- mN1A
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: Notch1 Antibody (mN1A) [TA336494] - Cell extracts from Jurkat (Lane 1) or mouse thymocytes (Lane 2) were Western Blot analyzed with monoclonal anti-NOTCH1 antibody. The mN1A antibody recognizes both mouse and human 270 kD full-length NOTCH1
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Notch1 Antibody (mN1A) [TA336494] - The Notch1 Antibody was tested in HEK293 cells at a 1:50 dilution against Dylight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mounted in DAP
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry: Notch1 Antibody (mN1A) [TA336494] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using Notch1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
- Validation comment
- FC
