845202

antibody from BioLegend

Targeting: NOTCH1 TAN1

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

845202

Provider

BioLegend

Proper citation

BioLegend Cat#845202, RRID:AB_2616641

Product name

Purified anti-Notch1β

Antibody type

Monoclonal

Antigen

KLH conjugated peptide corresponding to aa 1721-1745 of human Notch1 (VQSETVEPPPPAQLHFMYVAAAAFV)

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

5B6

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunohistochemistry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

IHC staining of clone 5B6 on formalin-fixed paraffin-embedded human stomach tissue at 1 µg/ml. Following Sodium Citrate antigen retrieval, the tissue was incubated with the primary antibody for 24 hours at 2-8°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) was used for detection.

Conjugate

Unconjugated