845402

antibody from BioLegend

Targeting: NOTCH1 TAN1

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

845402

Provider

BioLegend

Proper citation

BioLegend Cat#845402, RRID:AB_2616643

Product name

Purified anti-Notch1β

Antibody type

Monoclonal

Antigen

KLH conjugated peptide corresponding to aa 1721-1745 of human Notch1 (VQSETVEPPPPAQLHFMYVAAAAFV)

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

5C12

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunohistochemistry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

IHC staining of clone 5C12 on formalin-fixed paraffin-embedded human stomach tissue at 10 µg/ml. Following Sodium Citrate antigen retrieval, the tissue was incubated with the primary antibody for 24 hours at 2-8°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) was used for detection.

Conjugate

Unconjugated