PAB10291

antibody from Abnova Corporation

Targeting: NOTCH1 TAN1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10291

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10291, RRID:AB_1676310

Product name

NOTCH1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of NOTCH1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Notch signaling and inherited disease syndromes.

Gridley T
Human molecular genetics 2003 Apr 1;12 Spec No 1:R9-13

---

Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells.

Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW
Leukemia 2000 Dec;14(12):2095-102

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoblotting of NOTCH1 polyclonal antibody (Cat # PAB10291) was used at a 1 : 500 dilution to detect mouse NOTCH1 by Western blot.Equivalent amounts of lysates from transiently transfected 293 cells expressing recombinant myc-tagged mouse Notch constructs were electrophoresed and transferred to membrane using standard methods.A reaction with diluted primary antibody was followed by washing, reaction with a 1 : 10,000 dilution of HRP conjugated Gt-a-Rabbit IgG, and color development.Lane 1 : No transfection.Lane 2 : N1 (mouse deleted extracellular domain)-myc.Lane 3 : N1 (mouse intracellular domain)-myc.Lane 4 : N2 (mouse deleted extracellular domain)-myc.Lane 5 : N2 (mouse intracellular domain)-myc.Lane 6 : N3 (mouse deleted extracellular domain)-myc.Lane 7 : N3 (mouse intracellular domain)-myc.Lane 8 : N4 (mouse deleted extracellular domain)-myc.Lane 9 : N4 (mouse intracellular domain)-myc.Lane 10 : N1 (mouse deleted extracellular domain) (V to G)-myc.Personal communication, Dr. Stacey Huppert.