17-9889-42

antibody from Invitrogen Antibodies

Targeting: NOTCH1 TAN1

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17-9889-42

Provider

Invitrogen Antibodies

Product name

NOTCH1 Monoclonal Antibody (MHN1-519), APC, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This MHN1-519 monoclonal antibody reacts with human Notch1, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription.

Antibody clone number

MHN1-519

Concentration

5 µL/Test

References

Submitted references

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Menon V, Thomas R, Elgueta C, Horl M, Osborn T, Hallett PJ, Bartos M, Isacson O, Pruszak J
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306

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NOTCH1 mediates a switch between two distinct secretomes during senescence.

Hoare M, Ito Y, Kang TW, Weekes MP, Matheson NJ, Patten DA, Shetty S, Parry AJ, Menon S, Salama R, Antrobus R, Tomimatsu K, Howat W, Lehner PJ, Zender L, Narita M
Nature cell biology 2016 Sep;18(9):979-92

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Notch signaling in the immune system.

Radtke F, Fasnacht N, Macdonald HR
Immunity 2010 Jan 29;32(1):14-27

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Notch activation induces the generation of functional NK cells from human cord blood CD34-positive cells devoid of IL-15.

Haraguchi K, Suzuki T, Koyama N, Kumano K, Nakahara F, Matsumoto A, Yokoyama Y, Sakata-Yanagimoto M, Masuda S, Takahashi T, Kamijo A, Takahashi K, Takanashi M, Okuyama Y, Yasutomo K, Sakano S, Yagita H, Kurokawa M, Ogawa S, Chiba S
Journal of immunology (Baltimore, Md. : 1950) 2009 May 15;182(10):6168-78

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Direct regulation of Gata3 expression determines the T helper differentiation potential of Notch.

Amsen D, Antov A, Jankovic D, Sher A, Radtke F, Souabni A, Busslinger M, McCright B, Gridley T, Flavell RA
Immunity 2007 Jul;27(1):89-99

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TAN-1, the human homolog of the Drosophila notch gene, is broken by chromosomal translocations in T lymphoblastic neoplasms.

Ellisen LW, Bird J, West DC, Soreng AL, Reynolds TC, Smith SD, Sklar J
Cell 1991 Aug 23;66(4):649-61

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Normal human peripheral blood cells were either unstimulated (blue histogram) or stimulated with immobilized Anti-Human CD3 Functional Grade Purified (Product # 16-0039-81) for 24 hours (purple histogram) and then stained with Anti-Human Notch1 APC. Cells in the lymphocyte gate were used for analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Plasma membrane proteomics (PMP) defines NOTCH1 as upregulated in OIS. (a) The workflow for quantitative PMP using differential SILAC labelling of growing and HRAS G12V -induced senescent (RIS) IMR90 cells. (b) GO cellular compartment term enrichment for all 1502 identified proteins in both conditions. (c) Volcano plot of 521 high-confidence protein identifications from PMP demonstrating log 2 fold change (RIS(d6) / Growing) against negative log 10 p value (n = 4 independent experiments). Among 167 proteins differentially expressed during RIS (p