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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- NB300-251-0.1ml - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Notch-1 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. Notch-1 does not react with mouse N2, N3, or N4. It only recognizes the cleaved intracellular (activated) form (NICD).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antigen sequence
The epitope is only exposed after g
amma secretase cleavage and is not
accessible in the uncleaved form.- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Notch-1 Antibody [NB300-251] - Notch 1 Antibody [NB 300-251]- at 1:500, against myc-tagged transiently transfected mouse Notch constructs in 293 cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Notch-1 Antibody [NB300-251] - Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17beta-estradiol. Lane 3: MCF-7 + 10 uM gamma secretase inhibitor. Load: 35 ug per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80 kDa for Notch1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Notch-1 Antibody [NB300-251] - Analysis of Tissue: Exocrine glands of human pancreas Fixation: FFPE Primary antibody: Notch1 antibody at 1:200 Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Dot Blot: Notch-1 Antibody [NB300-251] - Dot Blot of Rabbit anti-Notch 1 (Cleaved N Terminal) (Human Specific) Antibody. Antigen: Row 1 - Notch 1 Peptide (Cleaved N Terminal) Row 2 - Notch 1 (Intra) Peptide. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: Rabbit anti-Notch 1 (Cleaved N Terminal) (Human Specific) Antibody at 1:1,000 for 60 min at RT. Secondary antibody: HRP Rabbit Secondary at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 1 HR at RT.
