Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 629103 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#629103, RRID:AB_493298
- Product name
- Biotin anti-Notch 1
- Antibody type
- Monoclonal
- Antigen
- Notch1 GST fusion protein
- Description
- Notch1, also known as neurogenic locus notch homolog protein 1, is a large >270 kD protein that functions as a receptor for the membrane ligands Jagged1, Jagged2 and Delta1 to regulate cell fate decisions. Upon ligand activation, the transmembrane Notch1 receptor is cleaved by TNF-alpha converting enzyme (TACE) to produce a membrane-associated intermediate fragment (NEXT). This fragment is further cleaved by presenilin-dependent gamma secretase to release a notch-derived peptide containing the intracellular fragment from the membrane. The released Notch intracellular fragment (NICD) translocates to the nucleus and forms a transcriptional complex with the RBP-J κ transcriptional activator complex to alter differentiation, proliferation, and apoptotic programs. Notch 1 is highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells) with lower levels of expression observed in the spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney and heart. The transmembrane Notch protein is a heterodimeric protein consisting of a C-terminal fragment and N-terminal fragment (probably linked by disulphide bonds) containing 5 ankyrin repeats, 36 EGF repeats, and 3 Notch/Lin repeats. Notch1 can be modified by phosphorylation. The mN1A monoclonal antibody reacts with the intracellular domain of mouse and human Notch1 and has been reported to have highest affinity for activated intracellular Notch1 and lower affinity for full-length unprocessed/heterodimeric Notch1 forms. This antibody does not recognize rat Notch1 or cross-react with Notch2, 3, or 4.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C. Do not freeze.
Submitted references Seventeen-gene signature from enriched Her2/Neu mammary tumor-initiating cells predicts clinical outcome for human HER2+:ERα- breast cancer.
Aberrant expression of Notch1 interferes with the B-lymphoid phenotype of neoplastic B cells in classical Hodgkin lymphoma.
Stra13 regulates satellite cell activation by antagonizing Notch signaling.
Liu JC, Voisin V, Bader GD, Deng T, Pusztai L, Symmans WF, Esteva FJ, Egan SE, Zacksenhaus E
Proceedings of the National Academy of Sciences of the United States of America 2012 Apr 10;109(15):5832-7
Proceedings of the National Academy of Sciences of the United States of America 2012 Apr 10;109(15):5832-7
Aberrant expression of Notch1 interferes with the B-lymphoid phenotype of neoplastic B cells in classical Hodgkin lymphoma.
Jundt F, Acikgöz O, Kwon SH, Schwarzer R, Anagnostopoulos I, Wiesner B, Mathas S, Hummel M, Stein H, Reichardt HM, Dörken B
Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K 2008 Aug;22(8):1587-94
Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K 2008 Aug;22(8):1587-94
Stra13 regulates satellite cell activation by antagonizing Notch signaling.
Sun H, Li L, Vercherat C, Gulbagci NT, Acharjee S, Li J, Chung TK, Thin TH, Taneja R
The Journal of cell biology 2007 May 21;177(4):647-57
The Journal of cell biology 2007 May 21;177(4):647-57
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with Biotin anti-Notch 1 Antibody (clone mN1A). Proteins were visualized using a Streptavidin-HRP and chemiluminescence detection.
- Conjugate
- Biotin