MA5-11961

antibody from Invitrogen Antibodies

Targeting: NOTCH1 TAN1

Western blot

Antibody data

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Validation data

Product number: MA5-11961 - Provider product page
Provider: Invitrogen Antibodies
Product name: NOTCH1 Monoclonal Antibody (A6)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: MA5-11961 targets Notch-1 in WB, FACS and ICC/IF applications and shows reactivity with Human and mouse samples. The MA5-11961 immunogen is recombinant protein encoding the ligand binding region of human Notch-1.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: A6
Vial size: 500 µL
Concentration: 0.2 mg/mL
Storage: 4° C

NOTCH1 protein structure - MA5-11961 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Notch-1 (green) showing staining in the membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Notch-1 monoclonal antibody (Product # MA5-11961) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Notch-1 (green) showing staining in the membrane of MCF-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Notch-1 monoclonal antibody (Product # MA5-11961) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Notch-1 (green) showing staining in the membrane of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Notch-1 monoclonal antibody (Product # MA5-11961) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.