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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NBP1-78292 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-78292, RRID:AB_11031502
- Product name
- Rabbit Polyclonal Notch-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.18 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Enhanced lysosomal degradation maintains the quiescent state of neural stem cells.
The periodontal pathogen Porphyromonas gingivalis changes the gene expression in vascular smooth muscle cells involving the TGFbeta/Notch signalling pathway and increased cell proliferation.
Mulberry leaf extract inhibits cancer cell stemness in neuroblastoma.
Kobayashi T, Piao W, Takamura T, Kori H, Miyachi H, Kitano S, Iwamoto Y, Yamada M, Imayoshi I, Shioda S, Ballabio A, Kageyama R
Nature communications 2019 Nov 29;10(1):5446
Nature communications 2019 Nov 29;10(1):5446
The periodontal pathogen Porphyromonas gingivalis changes the gene expression in vascular smooth muscle cells involving the TGFbeta/Notch signalling pathway and increased cell proliferation.
Zhang B, Elmabsout AA, Khalaf H, Basic VT, Jayaprakash K, Kruse R, Bengtsson T, Sirsjö A
BMC genomics 2013 Nov 9;14:770
BMC genomics 2013 Nov 9;14:770
Mulberry leaf extract inhibits cancer cell stemness in neuroblastoma.
Park S, Kim J, Kim Y
Nutrition and cancer 2012 Aug;64(6):889-98
Nutrition and cancer 2012 Aug;64(6):889-98
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Notch-1 Antibody [NBP1-78292] - Lane view shows a specific band for Notch-1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Notch-1 Antibody [NBP1-78292] - Analysis of Cleaved Notch1 in Jurkat cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Notch-1 Antibody [NBP1-78292] - Mouse total protein lysates (50 ug) were separated on 8% SDS-PAGE. Notch-1 (NBP1-78292SS) antibody was used at 1:500 dilution. Actin (1:10,000) was used as normalizer. WB image submitted by a verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Notch-1 Antibody [NBP1-78292] - Staining of Notch1 in human kidney carcinoma using DAB with hematoxylin counterstain.
