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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA5-32522 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOTCH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is breast or ovarian carcinoma.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Aberrant expression of semaphorin 6B affects cell phenotypes in thyroid carcinoma by activating the Notch signalling pathway.
Lv XJ, Chen X, Wang Y, Yu S, Pang L, Huang C
Endokrynologia Polska 2021;72(1):29-36
Endokrynologia Polska 2021;72(1):29-36
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of Notch-1 using anti-Notch-1 Polyclonal Antibody (Product # PA5-32522) in Ovarian Carcinoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:200.
