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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- NBP1-19751 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-19751, RRID:AB_1641505
- Product name
- Rabbit Polyclonal BMP-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.15 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Interleukin-1β-Induced Senescence Promotes Osteoblastic Transition of Vascular Smooth Muscle Cells.
18F-Choline PET/CT Identifies High-Grade Prostate Cancer Lesions Expressing Bone Biomarkers.
The nuclear variant of bone morphogenetic protein 2 (nBMP2) is expressed in macrophages and alters calcium response.
Influence of omega-3 fatty acid on orthodontic tooth movement in rats: A biochemical, histological, immunohistochemical and gene expression study.
Bone morphogenetic protein 2 is a depot-specific regulator of human adipogenesis.
Histomorphometry of ectopic mineralization using undecalcified frozen bone sections.
Prolonged release of bone morphogenetic protein-2 in vivo by gene transfection with DNA-functionalized calcium phosphate nanoparticle-loaded collagen scaffolds.
Bone Morphogenetic Proteins and myostatin pathways: key mediator of human sarcopenia.
Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel Enhances Bone Regeneration in Mice Cranial Bone Defect Model.
The BMP2 nuclear variant, nBMP2, is expressed in mouse hippocampus and impacts memory.
Reduced Dentin Matrix Protein Expression in Camurati-Engelmann Disease Transgenic Mouse Model.
Increased hepcidin in transferrin-treated thalassemic mice correlates with increased liver BMP2 expression and decreased hepatocyte ERK activation.
Satellite Cells CD44 Positive Drive Muscle Regeneration in Osteoarthritis Patients.
Effects of laser acupuncture on longitudinal bone growth in adolescent rats.
A novel bone morphogenetic protein 2 mutant mouse, nBmp2NLS(tm), displays impaired intracellular Ca2+ handling in skeletal muscle.
Effect of sodium nitrate loading on electrolyte transport by the renal tubule.
Han L, Zhang Y, Zhang M, Guo L, Wang J, Zeng F, Xu D, Yin Z, Xu Y, Wang D, Zhou H
Kidney & blood pressure research 2020;45(2):314-330
Kidney & blood pressure research 2020;45(2):314-330
18F-Choline PET/CT Identifies High-Grade Prostate Cancer Lesions Expressing Bone Biomarkers.
Urbano N, Scimeca M, Crocco A, Mauriello A, Bonanno E, Schillaci O
Journal of clinical medicine 2019 Oct 11;8(10)
Journal of clinical medicine 2019 Oct 11;8(10)
The nuclear variant of bone morphogenetic protein 2 (nBMP2) is expressed in macrophages and alters calcium response.
Tellez Freitas CM, Burrell HR, Valdoz JC, Hamblin GJ, Raymond CM, Cox TD, Johnson DK, Andersen JL, Weber KS, Bridgewater LC
Scientific reports 2019 Jan 30;9(1):934
Scientific reports 2019 Jan 30;9(1):934
Influence of omega-3 fatty acid on orthodontic tooth movement in rats: A biochemical, histological, immunohistochemical and gene expression study.
Ogrenim G, Cesur MG, Onal T, Kara M, Sirin FB, Yalcin GD, Inan S
Orthodontics & craniofacial research 2019 Feb;22(1):24-31
Orthodontics & craniofacial research 2019 Feb;22(1):24-31
Bone morphogenetic protein 2 is a depot-specific regulator of human adipogenesis.
Denton NF, Eghleilib M, Al-Sharifi S, Todorčević M, Neville MJ, Loh N, Drakesmith A, Karpe F, Pinnick KE
International journal of obesity (2005) 2019 Dec;43(12):2458-2468
International journal of obesity (2005) 2019 Dec;43(12):2458-2468
Histomorphometry of ectopic mineralization using undecalcified frozen bone sections.
Fujihara R, Chiba Y, Nakagawa T, Murakami R, Matsumoto K, Kawauchi M, Fujii T, Shimono R, Yamamoto T, Ueno M
Microscopy research and technique 2018 Nov;81(11):1318-1324
Microscopy research and technique 2018 Nov;81(11):1318-1324
Prolonged release of bone morphogenetic protein-2 in vivo by gene transfection with DNA-functionalized calcium phosphate nanoparticle-loaded collagen scaffolds.
Tenkumo T, Vanegas Sáenz JR, Nakamura K, Shimizu Y, Sokolova V, Epple M, Kamano Y, Egusa H, Sugaya T, Sasaki K
Materials science & engineering. C, Materials for biological applications 2018 Nov 1;92:172-183
Materials science & engineering. C, Materials for biological applications 2018 Nov 1;92:172-183
Bone Morphogenetic Proteins and myostatin pathways: key mediator of human sarcopenia.
Scimeca M, Piccirilli E, Mastrangeli F, Rao C, Feola M, Orlandi A, Gasbarra E, Bonanno E, Tarantino U
Journal of translational medicine 2017 Feb 15;15(1):34
Journal of translational medicine 2017 Feb 15;15(1):34
Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin-PLGA Hydrogel Enhances Bone Regeneration in Mice Cranial Bone Defect Model.
Sivashanmugam A, Charoenlarp P, Deepthi S, Rajendran A, Nair SV, Iseki S, Jayakumar R
ACS applied materials & interfaces 2017 Dec 13;9(49):42639-42652
ACS applied materials & interfaces 2017 Dec 13;9(49):42639-42652
The BMP2 nuclear variant, nBMP2, is expressed in mouse hippocampus and impacts memory.
Cordner RD, Friend LN, Mayo JL, Badgley C, Wallmann A, Stallings CN, Young PL, Miles DR, Edwards JG, Bridgewater LC
Scientific reports 2017 Apr 18;7:46464
Scientific reports 2017 Apr 18;7:46464
Reduced Dentin Matrix Protein Expression in Camurati-Engelmann Disease Transgenic Mouse Model.
Gullard A, Croney CM, Wu X, Mamaeva O, Sohn P, Cao X, MacDougall M
Journal of cellular physiology 2016 May;231(5):1106-13
Journal of cellular physiology 2016 May;231(5):1106-13
Increased hepcidin in transferrin-treated thalassemic mice correlates with increased liver BMP2 expression and decreased hepatocyte ERK activation.
Chen H, Choesang T, Li H, Sun S, Pham P, Bao W, Feola M, Westerman M, Li G, Follenzi A, Blanc L, Rivella S, Fleming RE, Ginzburg YZ
Haematologica 2016 Mar;101(3):297-308
Haematologica 2016 Mar;101(3):297-308
Satellite Cells CD44 Positive Drive Muscle Regeneration in Osteoarthritis Patients.
Scimeca M, Bonanno E, Piccirilli E, Baldi J, Mauriello A, Orlandi A, Tancredi V, Gasbarra E, Tarantino U
Stem cells international 2015;2015:469459
Stem cells international 2015;2015:469459
Effects of laser acupuncture on longitudinal bone growth in adolescent rats.
Yeom M, Kim SH, Lee B, Zhang X, Lee H, Hahm DH, Sohn Y, Lee H
Evidence-based complementary and alternative medicine : eCAM 2013;2013:424587
Evidence-based complementary and alternative medicine : eCAM 2013;2013:424587
A novel bone morphogenetic protein 2 mutant mouse, nBmp2NLS(tm), displays impaired intracellular Ca2+ handling in skeletal muscle.
Bridgewater LC, Mayo JL, Evanson BG, Whitt ME, Dean SA, Yates JD, Holden DN, Schmidt AD, Fox CL, Dhunghel S, Steed KS, Adam MM, Nichols CA, Loganathan SK, Barrow JR, Hancock CR
BioMed research international 2013;2013:125492
BioMed research international 2013;2013:125492
Effect of sodium nitrate loading on electrolyte transport by the renal tubule.
Kahn T, Bosch J, Levitt MF, Goldstein MH
The American journal of physiology 1975 Sep;229(3):746-53
The American journal of physiology 1975 Sep;229(3):746-53
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BMP-2 Antibody [NBP1-19751] - Extracts from HUVEC cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BMP-2 Antibody [NBP1-19751] - Protein expression analysis. Immunostaining for BMP-2, myostatin, and CD44 was evaluated by counting the number of positive fibers/cells on 25-high power field (HPF), whereas western blot for BMP-2 was evaluated by lines densitometry. Western blot lines show a higher expression of BMP-2 in OA patients compared to OP group. Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/sci/2015/469459/), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: BMP-2 Antibody [NBP1-19751] - Analysis of BMP2 in mouse heart using DAB with hematoxylin counterstain.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BMP-2 Antibody [NBP1-19751] - Paraffin-embedded human heart tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BMP-2 Antibody [NBP1-19751] - Molecular analysis of BMP-2 expression. In order to verify the correlation between BMP-2 and satellite cells activity we performed both western blot and immune-gold analysis. In muscle biopsies of OA patients we found several large satellite cells syncytium (arrows). High magnifications show immunolabeling for BMP-2 in perinuclear areas (squares) (40.000x). Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/sci/2015/469459/), licensed under a CC-BY licence.
