Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-76711 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Plasma Kallikrein Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Plasma Kallikrein as a Modulator of Liver Injury/Remodeling.
Ahmed IA, Jaffa MA, Moussa M, Hatem D, El-Achkar GA, Al Sayegh R, Karam M, Hamade E, Habib A, Jaffa AA
Frontiers in pharmacology 2021;12:715111
Frontiers in pharmacology 2021;12:715111
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Plasma Kallikrein. Samples were incubated with Plasma Kallikrein polyclonal antibody (Product # PA5-76711).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 2 Klkb1 gene expression is translated to the pre-kallikrein protein and stimulates cell proliferation. (A) Representative images of immunostaining of plasma kallikrein (black arrows; dark red clusters; original magnification x40 (top) and x100 (bottom)) at days 1, 2, and 3 of liver injury after the CCl 4 or vehicle (mineral oil) injection. (B) Percentage viability of HepG2 treated 1 or 2 mM CCl 4 or 2.5 ng/ml plasma kallikrein (PKK) for 24 h ( n = 8). (C) Percentage viability and (D) proliferation of plasma kallikrein-treated cells for 1 or 2 days ( n = 8). Data are shown as mean +- SEM. Statistical significance was determined by one-way ANOVA followed by Sidak's multiple comparisons, where * p < 0.05, ** p < 0.005 (PKK vs. control,), *** p < 0.0005 (1 mM CCl 4 vs. control; 2 mM CCl 4 vs. 1mM CCl 4 ), **** p < 0.0001 (Day 2-PKK vs. others; 1mM CCl 4 +PKK vs. 1mM CCl 4 ). (E) Plasma kallikrein (PKK) increased the mRNA levels of F2R, and IL1B genes ( n = 8). Data are shown as mean +- SEM. Statistical significance was determined by the Mann-Whitney test, * p < 0.05 (PKK versus CTL). CTL corresponds to the control group with 0.5 % DMSO as vehicle.