Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
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Validation data
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- Product number
- LS-C20223 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-C20223, RRID:AB_2210897
- Product name
- UBE2L3 / UBCH7 Antibody (aa110-122) LS-C20223
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Horse, Porcine, Rabbit, Simian, Xenopus
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Staining (0.5 ug/ml) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Goat Anti-UBE2L3 Antibody (0.01µg/ml) staining of HEK293 (A), HepG2 (B) and Jurkat (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Staining (0.5 ug/ml) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Goat Anti-UBE2L3 Antibody (0.01µg/ml) staining of HEK293 (A), HepG2 (B) and Jurkat (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Goat Anti-UBE2L3 Antibody (0.01µg/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Goat Anti-UBE2L3 Antibody (0.03µg/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.