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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- M01204-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Fibrinogen beta chain/FGB Antibody Picoband™ (monoclonal, 6D12)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for Fibrinogen beta chain/FGB detection. Tested with WB, IHC-P in Human.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6D12
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Pleural thickening induced by Glaesserella parasuis infection was linked to increased collagen and elastin.
Gong H, Chen L, He Y, Hua K, Ma B, Gao Y, Xu X, Hu X, Jin H
Frontiers in cellular and infection microbiology 2022;12:952377
Frontiers in cellular and infection microbiology 2022;12:952377
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Fibrinogen beta chain/FGB using anti-Fibrinogen beta chain/FGB antibody (M01204-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Fibrinogen beta chain/FGB antigen affinity purified monoclonal antibody (Catalog # M01204-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Fibrinogen beta chain/FGB at approximately 56KD. The expected band size for Fibrinogen beta chain/FGB is at 56KD.
- Additional image
