PA5-29805

antibody from Invitrogen Antibodies

Targeting: FGB

Antibody data

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Validation data

Product number: PA5-29805 - Provider product page
Provider: Invitrogen Antibodies
Product name: FIBB Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: human plasma, mouse plasma, rat plasma, human platelet. Predicted reactivity: Mouse (91%), Rat (90%), Xenopus laevis (81%), Pig (88%), Chicken (82%), Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.13 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

FGB protein structure - PA5-29805 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Fibrinogen beta using 50 µg of mouse liver lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Fibrinogen beta polyclonal antibody (Product # PA5-29805) at a dilution of 1:5000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Fibrinogen beta using 30 µg of MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Fibrinogen beta polyclonal antibody (Product # PA5-29805) at a dilution of 1:5000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of FIBB was performed by separating 30 µg of human tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a FIBB Polyclonal Antibody (Product # PA5-29805) at a dilution of 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using FIBB Polyclonal Antibody (Product # PA5-29805). Human plasma (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with FIBB Polyclonal Antibody (Product # PA5-29805) diluted at 1:50,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of FIBB was performed by separating 30 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a FIBB Polyclonal Antibody (Product # PA5-29805) at a dilution of 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of FIBB was performed by separating 30 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a FIBB Polyclonal Antibody (Product # PA5-29805) at a dilution of 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.