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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00002147-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002147-M01, RRID:AB_548661
- Product name
- Mouse Monoclonal Coagulation Factor II/Thrombin Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. F2 - coagulation factor II (thrombin)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Prothrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi ( Cat # H00002147-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with F2 monoclonal antibody (M01), clone 1B7 (Cat # H00002147-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Prothrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody (M01), clone 1B7.Lane 1: F2 transfected lysate(70 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Prothrombin Antibody (1B7) [H00002147-M01] - Detection limit for recombinant GST tagged F2 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Prothrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 transfected lysate using anti-F2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F2 MaxPab rabbit polyclonal antibody.
