MAB4976

antibody from Novus Biologicals

Targeting: PROS1 PROS

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB4976

Provider

Novus Biologicals

Product name

Rat Monoclonal Protein S/PROS1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse Protein S/PROS1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Protein S is observed.

Reactivity

Mouse

Host

Rat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Anticoagulant Protein S Targets the Factor IXa Heparin-Binding Exosite to Prevent Thrombosis.

Plautz WE, Sekhar Pilli VS, Cooley BC, Chattopadhyay R, Westmark PR, Getz T, Paul D, Bergmeier W, Sheehan JP, Majumder R
Arteriosclerosis, thrombosis, and vascular biology 2018 Apr;38(4):816-828

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse Protein S/PROS1 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Protein S/PROS1 Monoclonal Antibody (Catalog # MAB4976) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Protein S/PROS1 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.