LS-B403

antibody from LSBio

Targeting: A2M CPAMD5, FWP007, S863-7

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B403

Provider

LSBio

Product name

IHC-plus™ A2M / Alpha-2-Macroglobulin Antibody LS-B403

Antibody type

Polyclonal

Description

Purified IgG

Reactivity

Human

Host

Goat

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Alpha-2-Macroglobulin Polyclonal Antibody-Western blot. Goat anti-Alpha-2-Macroglobulin antibody was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti-goat (1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.