TA319295

antibody from OriGene

Targeting: A2M CPAMD5, FWP007, S863-7

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319295

Provider

OriGene

Product name

Goat polyclonal Alpha-2-Macroglobulin antibody

Antibody type

Polyclonal

Description

Goat polyclonal Alpha-2-Macroglobulin antibody

Host

Goat

Conjugate

Unconjugated

Epitope

A2M

Antibody clone number

NULL

Vial size

2 ml

Concentration

90 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot of Goat anti-Alpha-2-Macroglobulin Antibody. Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight„? 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Goat anti Alpha-2-Macroglobulin (200-101-207 lot 20172) was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti goat.

Validation comment

WB