TA319296

antibody from OriGene

Targeting: A2M CPAMD5, FWP007, S863-7

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319296

Provider

OriGene

Product name

Goat polyclonal Alpha-2-Macroglobulin antibody

Antibody type

Polyclonal

Description

Goat polyclonal Alpha-2-Macroglobulin antibody

Host

Goat

Conjugate

Unconjugated

Epitope

A2M

Antibody clone number

NULL

Vial size

100 µg

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot of Goat anti-Alpha-2-Macroglobulin Antibody. Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight„? 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Goat anti Alpha-2-Macroglobulin antibody was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat.

Validation comment

WB