Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- NB120-7339-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal alpha 2-Macroglobulin Antibody
- Antibody type
- Polyclonal
- Description
- Ion exchange chromatography.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Horseradish Peroxidase
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: alpha 2-Macroglobulin Antibody [HRP] [NB120-7339] - Detection of Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.