ABIN457943

antibody from antibodies-online

Targeting: A2M CPAMD5, FWP007, S863-7

No application information provided.

Antibody Data

Product number

ABIN457943

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN457943, RRID:AB_10780603

Product name

anti-alpha-2-Macroglobulin (A2M) antibody

Antibody type

Polyclonal

Antigen

Alpha-2 macroglobulin(clotting factor I) is the major protein in the alpha-2 region of human serum after separation by immunoelectrophoresis. The concentration in normal plasma is about 2.5 mg/ml. It is a large polymer and a strong antigen. It forms about one third in the total of the alpha-2 globulins. The protein is isolated by ammonium sulphate precipitation, ion exchange chromatography, gelfiltration and preparative starch block electrophoresis. In the purification the activation of kallikrein is avoided, since it forms an irreversible bond with alpha-2 macroglobulin, thus contaminating the final immunogen. Freund?s complete adjuvant is used in the first step of the immunization procedure.

Description

Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

1 mL

Concentration

Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. Antibody titre: Precipitin titre 1:64 when tested against pooled normal human plasma in agar-block immunodiffusion titration. No foreign proteins ad

Storage

4°C