200-101-456

antibody from Rockland Immunochemicals, Inc.

Targeting: AHSG A2HS, FETUA, HSGA

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

200-101-456

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#200-101-456, RRID:AB_2224118

Product name

Anti-Fetuin (2-HS Glycoprotein) (GOAT) Antibody - 200-101-456

Antibody type

Polyclonal

Vial size

500 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot of Fetuin. Anti-Human Fetuin antibody, generated by immunization with mature protein, was tested by western blot against fetuin in purified preparations and in human plasma. Lane 1 contains 250 ng of purified human fetuin. Lane 2 contains 5 ?l of a 1:50 dilution of human serum. Dilution of Anti-Human Fetuin antibody between 1:10,000 and 1:20,000 showed strong reactivity by western blot. In this blot the antibody was used at a 1:10,000 dilution incubated 1 h at room temperature in 1% BSA in TTBS. Detection occurred using a 1:5,000 dilution of IRDye™800 conjugated Donkey anti-Goat IgG (code # 605-732-125) for 45 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Validation comment

Western Blot