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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 209-4134-0100 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#209-4134-0100, RRID:AB_2612780
- Product name
- Anti-TRANSFERRIN (Human Serum) (RABBIT) Antibody - 209-4134-0100
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of primary and Dylight conjugated secondary antibodies. Lane 1: molecular weight. Lane 2: Rabbit anti-Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Primary antibody: Each primary antibody was diluted to 1:1000 for 2 hrs at RT. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight™ 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight™ 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight™ 488 Conjugated (blue)secondary antibody at 1:10,000 for 45 min at RT. Block: IRdye blocking buffer (MB-070) 1hr at 4°C. Other band(s): none.
- Validation comment
- Western Blot
