109-4134

antibody from Rockland Immunochemicals, Inc.

Targeting: TF PRO1557, PRO2086

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

109-4134

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#109-4134, RRID:AB_2255863

Product name

Anti-TRANSFERRIN (Human Serum) (RABBIT) Antibody - 109-4134

Antibody type

Polyclonal

Vial size

2 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Rockland primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin (1° 109-4134, green 2° 611-743-127); Alpha 1 anti trypsin (1° 100-101-147, red 2° 605-742-125); and Human IgG (1° 109-3102, Blue 2° 610-741-124 in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in IRdye blocking buffer (MB-070) and incubated for 2 hrs at RT. Blot was 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in IRdye blocking buffer and incubated ~ 1hr at 4 degrees. After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged using the Biorad VersaDoc4000.

Validation comment

Western Blot