A04700

antibody from Boster Biological Technology

Targeting: CASP12

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A04700

Provider

Boster Biological Technology

Product name

Anti-Caspase-12 Casp12 Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal antibody for Casp12 detection. Tested positive for IHC, WB in Human, Mouse, Rat

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100µg

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

+4°C

References

Submitted references

Effect of endoplasmic reticulum stress involved in manganese‑induced neurotoxicity in rats.

Wu C, Yuan G, Mo R, Huang Y, Luo T, Wang J
Molecular medicine reports 2019 Jun;19(6):5169-5176

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of Casp12 using anti-Casp12 antibody (A04700).Casp12 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Casp12 Antibody (A04700) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image